Difference between revisions of "Team:MSU-Michigan/IPTG Induction"

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<p>Induction of mtrB GFP measured using a potentiostat under anaerobic conditions in a single cell bioreactor.  The sample was induced with 200uM IPTG at 1.98 hours.</p>
 
<p>Induction of mtrB GFP measured using a potentiostat under anaerobic conditions in a single cell bioreactor.  The sample was induced with 200uM IPTG at 1.98 hours.</p>
 
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Revision as of 20:52, 24 October 2017

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IPTG Induction

IPTG Induction of various strains of Shewanella Oneidnensis.

M5 Media Fluorescence

Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.

mtrB Induction Fluorescence

Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control

mtrB_GFP Induction Fluorescence

Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. This is the highest because resources are not being directed toward the mtrB pathway, which is still removed

mrtB_GFP_mtrB Induction Fluorescence

Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.

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mtrB GFP Induction Current

Induction of mtrB GFP measured using a potentiostat under anaerobic conditions in a single cell bioreactor. The sample was induced with 200uM IPTG at 1.98 hours.