Difference between revisions of "Team:UNOTT/Demonstrate"

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<h3>★ ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<p><span style="color: #ffffff;">&nbsp;</span></p>
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<p><span style="color: #ffffff;">&nbsp;</span></p>
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<h1 style="text-align: center;"><span style="color: #ffffff;">Demonstrate:PROOF OF CONCEPT</span></h1>
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<p><span style="color: #ffffff;">&nbsp;</span></p>
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<p><span style="color: #ffffff;">Our first piece of evidence that we are likely to get many combinations from our plasmid arrangements is the following image:</span></p>
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<center><img src="https://static.igem.org/mediawiki/2017/9/9c/T--UNOTT--variationincolours.jpeg"></center>
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<p><span style="color: #ffffff;">Within each quadrant, the colonies here have been transformed with exactly the same plasmid, yet generate different colours. We have not sequenced all of these colonies since transformation, however they were transformed with a sequenced plasmid. This made us realise that you could never replicate one of the bacteria we send as a key by simply sequencing and transforming the bacteria with this plasmid. This increases security of our system. In order to investigate this idea further, we wanted to check that once the bacteria have developed a colour, that level stays more or less the same. We took a colony containing P<sub>4</sub>-sRFP, resuspended it in water then pipetted out 3ul into each spot shown below. The consistency of these colours shows that our results are reproducible enough to maintain access to whatever it is you have locked, yet not reproducible enough that anyone with the plasmid sequence could gain access. Success!</span></p>
  
 
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Revision as of 14:45, 25 October 2017


 

 

Demonstrate:PROOF OF CONCEPT

 

Our first piece of evidence that we are likely to get many combinations from our plasmid arrangements is the following image:

Within each quadrant, the colonies here have been transformed with exactly the same plasmid, yet generate different colours. We have not sequenced all of these colonies since transformation, however they were transformed with a sequenced plasmid. This made us realise that you could never replicate one of the bacteria we send as a key by simply sequencing and transforming the bacteria with this plasmid. This increases security of our system. In order to investigate this idea further, we wanted to check that once the bacteria have developed a colour, that level stays more or less the same. We took a colony containing P4-sRFP, resuspended it in water then pipetted out 3ul into each spot shown below. The consistency of these colours shows that our results are reproducible enough to maintain access to whatever it is you have locked, yet not reproducible enough that anyone with the plasmid sequence could gain access. Success!