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<h1>Measurement</h1>
 
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<p>MSU-iGEM 2017 developed a measurement tool that researchers throughout synthetic biology can utilize. Green fluorescent proteins (GFP) are used under aerobic conditions to signal protein expression. Unfortunately, for researchers working under anaerobic conditions, GFP does not mature and thus fluoresce under anaerobic conditions. Therefore, we showed that current induced via IPTG produces a reproducible signal for protein expression under anaerobic conditions. Current induction occurs 18 min after IPTG is added displaying a more rapid response than GFP expression. This measurement tool even proves to be more efficient than GFP expression under aerobic conditions due to the rapid current increased after induction. Also, current can be measured using simpler measuring tools than GFP expression. MSU-iGEM’s simplistic bioreactors utilizing Arduino boards are a model example of a system that can quantitatively measure protein expression via current increase in a more versatile way than using tools for measuring GFP fluorescence.</p>
  
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
 
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<h3>Best Innovation in Measurement Special Prize</h3>
 
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
 
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
 
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You must also delete the message box on the top of this page to be eligible for this prize.
 
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<h5>Inspiration</h5>
 
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<p>You can look at what other teams did to get some inspiration! <br />
 
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Here are a few examples:</p>
 
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
 
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<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
 
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
 
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
 
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Revision as of 18:40, 25 October 2017

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Measurement

MSU-iGEM 2017 developed a measurement tool that researchers throughout synthetic biology can utilize. Green fluorescent proteins (GFP) are used under aerobic conditions to signal protein expression. Unfortunately, for researchers working under anaerobic conditions, GFP does not mature and thus fluoresce under anaerobic conditions. Therefore, we showed that current induced via IPTG produces a reproducible signal for protein expression under anaerobic conditions. Current induction occurs 18 min after IPTG is added displaying a more rapid response than GFP expression. This measurement tool even proves to be more efficient than GFP expression under aerobic conditions due to the rapid current increased after induction. Also, current can be measured using simpler measuring tools than GFP expression. MSU-iGEM’s simplistic bioreactors utilizing Arduino boards are a model example of a system that can quantitatively measure protein expression via current increase in a more versatile way than using tools for measuring GFP fluorescence.