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The study required us to measure a number of different fluorescent test devices sent to us by iGEM HQ, we followed their protocol and then shared the results we obtain. Teams around the world do the same; collectively, we provide a large set of data illustrating how similar the results were, potentially highlighting any aspects in which the protocol could be improved and, hopefully, showing that this is possible. This is quite an important issue, we felt we should participate and help in any way we could! | The study required us to measure a number of different fluorescent test devices sent to us by iGEM HQ, we followed their protocol and then shared the results we obtain. Teams around the world do the same; collectively, we provide a large set of data illustrating how similar the results were, potentially highlighting any aspects in which the protocol could be improved and, hopefully, showing that this is possible. This is quite an important issue, we felt we should participate and help in any way we could! | ||
</span></center></p> | </span></center></p> | ||
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<center><h1>Methods</h1></center> | <center><h1>Methods</h1></center> | ||
− | <aside><img src="https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg" style="height:auto;width:350px;"></aside> | + | <aside><img src="https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg"aligh:"right" style="height:auto;width:350px;"></aside> |
<p><center>We were given 8 specimens to measure, termed “test devices”. These included a positive (BBa_I20270) and negative(BBa_R0040) control, and 6 different test devices (BBa_J36400, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005) . | <p><center>We were given 8 specimens to measure, termed “test devices”. These included a positive (BBa_I20270) and negative(BBa_R0040) control, and 6 different test devices (BBa_J36400, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005) . | ||
First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. | First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. |
Revision as of 20:41, 25 October 2017
![](https://static.igem.org/mediawiki/parts/0/09/Plates_for_background.jpeg)
INTERLAB STUDY:
![](https://2017.igem.org/File:Fluorescent_image.jpeg)
Introduction
Introduction
Methods
Results
LUDOX-S40 and Fluoroscein Reference Measurements
OD600nm and Fluorescence Results
µM Fluorescein/OD600 and Ln µM FITC/OD600 Results