MattAFrench (Talk | contribs) |
MattAFrench (Talk | contribs) |
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<center><h1>Methods</h1></center> | <center><h1>Methods</h1></center> | ||
− | <img src="https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg" ; style="height:auto;width:350px;" | + | <p><center><img src="https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg" ; style="height:auto;width:350px;" |
− | align:"right" | + | align:"right">We were given 8 specimens to measure, termed “test devices”. These included a positive (BBa_I20270) and negative(BBa_R0040) control, and 6 different test devices (BBa_J36400, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005). |
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First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. | First we transformed these into DH5α-E. coli cells, then selected two resulting colonies to be prepared for measurement. The two colonies were grown overnight. | ||
But before the experiment could be undertaken, we needed to create calibrate our equipment by performing standard reference measurements, enabling the conversion of our data to absolute values. Firstly, we measured a solution of half LUDOX-S40, half water; fluorescein was then serially diluted by half 10 times and measured to give a standard curve. | But before the experiment could be undertaken, we needed to create calibrate our equipment by performing standard reference measurements, enabling the conversion of our data to absolute values. Firstly, we measured a solution of half LUDOX-S40, half water; fluorescein was then serially diluted by half 10 times and measured to give a standard curve. |
Revision as of 20:46, 25 October 2017
![](https://static.igem.org/mediawiki/parts/0/09/Plates_for_background.jpeg)
INTERLAB STUDY:
![](https://2017.igem.org/File:Fluorescent_image.jpeg)
Introduction
Introduction
Methods
![](https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg)
Results
LUDOX-S40 and Fluoroscein Reference Measurements
OD600nm and Fluorescence Results
µM Fluorescein/OD600 and Ln µM FITC/OD600 Results