Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Methods"
| Line 54: | Line 54: | ||
<li><a href="https://static.igem.org/mediawiki/2017/d/d4/T--Bielefeld-CeBiTec--protocol_TAE_buffer.pdf" target="blank">TAE buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/d/d4/T--Bielefeld-CeBiTec--protocol_TAE_buffer.pdf" target="blank">TAE buffer</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--protocol_ZymoPURETM_PlasmidMiniprep_Kit.pdf" target="blank">ZymoPURE<sup>TM</sup> Plasmid Miniprep Kit (Zymo Research)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--protocol_ZymoPURETM_PlasmidMiniprep_Kit.pdf" target="blank">ZymoPURE<sup>TM</sup> Plasmid Miniprep Kit (Zymo Research)</a></li> | ||
| − | <li><a href=" | + | <li><a href="https://static.igem.org/mediawiki/2017/8/8c/T--Bielefeld-CeBiTec--YKE_lycopene_protocol.pdf" target="blank">Lycopene extraction</a></li> |
<li><a href="https://static.igem.org/mediawiki/2017/0/0d/T--Bielefeld-CeBiTec--protocol_SDS-PAGE.pdf" target="blank">SDS-PAGE</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/0/0d/T--Bielefeld-CeBiTec--protocol_SDS-PAGE.pdf" target="blank">SDS-PAGE</a></li> | ||
</ul> | </ul> | ||
Revision as of 14:14, 26 October 2017
Methods
Protocols
- 50xTAE Stock Solution
- Acetone Precipitation
- Biotynilation of glass slides
- Blunt end ligation
- Colony PCR GoTaq® G2 DNA Polymerase
- Transformation via electroporation
- Fast cell lysis sample buffer
- Fast cell lysis for SDS-PAGE
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- Generating chemocompetent cells
- Generating electrocompetent cells
- Gibson Assembly
- Transformation via heat shock
- innuPREP Gel Extraktion Kit (Analytik Jena)
- Lysogeny Broth (LB)
- Monarch® Plasmid Miniprep Kit (NEB)
- Monarch® PCR & DNA Cleanup Kit (NEB)
- MOPS Minimal Medium
- Native DNA PAGE
- PBJR buffer
- PCR with Q5 High-Fidelity Polymerase
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- PureYieldTM Plasmid Miniprep System (Promega)
- "Typical" Restriction Digest
- SDS-PAGE running buffer
- SOC-medium
- ssDNA Annealing
- Standard BioBrick Assembly
- TAE buffer
- ZymoPURETM Plasmid Miniprep Kit (Zymo Research)
- Lycopene extraction
- SDS-PAGE
Enzymes
Digestion enzymes
| Enzyme | Producer |
|---|---|
| AgeI-HF | NEB |
| BsaI | NEB |
| DpnI | NEB |
| DpnI | Jena Bioscience |
| EciI | NEB |
| EcoRV | Fermentas |
| MnlI | NEB |
| NotI | Jena Bioscience |
| PstI | NEB |
| PstI | Thermo Fisher Scientific |
| PstI-HF | NEB |
| SapI | NEB |
| SpeI | NEB |
| XbaI | NEB |
| XbaI | Jena Bioscience |
Polymerases
| Enzyme | Producer |
|---|---|
| Go Taq DNA polymerase | Promega |
| Klenow Fragment | Thermo Fisher Scientific |
| Phusion High-Fidelity DNA polymerase | NEB |
| Q5 High-Fidelity DNA polymerase | NEB |
| Taq Polymerase | Jena Bioscience |
Other Enzymes
| Enzyme | Producer |
|---|---|
| Shrimp Alkaline Phosphatase | Jena Bioscience |
| T4 DNA Ligase | NEB |
| T4 DNA Ligase | Thermo Fisher Scientific |
| T4 Polynucleotide Kinase | NEB |
