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− | {{NPU-China}} | + | {{NPU-China/mmp}} |
− | <html> | + | <html lang="en"> |
+ | <head> | ||
+ | <!-- Bootstrap Core CSS --> | ||
+ | <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet"> | ||
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− | </div> | + | <!-- Navigation --> |
+ | <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
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+ | <span class="icon-bar"></span> | ||
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+ | </button> | ||
+ | <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | ||
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China">Home</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="active"> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook | ||
+ | <b class="caret"></b> | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <!-- Page Content --> | ||
+ | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/e/e1/%E9%A2%98%E7%9B%AE%E5%B0%8F%E9%80%9A%E6%A0%8Fhardware.jpg"> | ||
+ | <div class="container" style="padding-top:50px"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | |||
+ | |||
+ | <h4>The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardware | ||
+ | to model the industrial production and enhance our method. However, the gap does exist between the | ||
+ | reality and our expectation. | ||
+ | </h4> | ||
+ | |||
+ | <h3 style="text-align:center">List of what we use</h3> | ||
+ | |||
+ | <h4>By listing the main items we have purchased and actually used, we hope to provide some experience and | ||
+ | inspiration for the future team wishing to have HW production. | ||
+ | </h4> | ||
+ | <div align="center"> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/19/Hardware_tab.png" width="60%" height="60%" > | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <h3 style="text-align:center">Hardware Design</h3> | ||
+ | |||
+ | <h4>Our system is mainly composed of two parts: reaction part and concentrated crystallization part. The | ||
+ | overall idea is that the reaction part provides the bacteria with appropriate reaction temperature, | ||
+ | adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the | ||
+ | reaction solution will be transferred to the concentrated crystallization part, where the concentration | ||
+ | of the productivity is increased by the reverse osmosis membrane; the temperature of the reaction | ||
+ | solution is lowered by the device, and the acrylic acid is finally crystallized. | ||
+ | </h4> | ||
+ | |||
+ | <h4>1)Reaction Part</h4> | ||
+ | |||
+ | <h4>The reaction part mainly uses miniature DC self-priming pump, foam machine, acrylic material container, | ||
+ | aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor. | ||
+ | </h4> | ||
+ | |||
+ | <h4> | ||
+ | (图)Fig1 Hontainer made of Acrylics Fig2 Hollow cylinder made of aluminum alloy Fig3Miniature DC self - priming pump Fig4 | ||
+ | 3D design drawing | ||
+ | </h4> | ||
+ | |||
+ | |||
+ | <h4>Instructions:When the system starts, the pump will continue to pump the upper reaction solution, which | ||
+ | will blend a large amount of air bubbles after passing the foam machine, and then will be pumped | ||
+ | back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and | ||
+ | the bubbles are continuously rising, the purpose of stirring the reaction solution and providing | ||
+ | sufficient air can be achieved. Simultaneously, with the help of the temperature sensor, the system | ||
+ | will be able to control heating wire inside the aluminum alloy cylinder, so that the reaction solution | ||
+ | can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials | ||
+ | is conducive to insulation. (We had considered heating the air mixed with the reaction solution at | ||
+ | the foaming machine so that the reaction liquid pumped back to the reaction vessel would be blended | ||
+ | with a large amount of hot air, which could replace the additional heating device, but due to the | ||
+ | safety and energy efficiency, this thought was given up.) | ||
+ | </h4> | ||
+ | |||
+ | <h4>2)Concentrated Crystallization Part</h4> | ||
+ | |||
+ | <h4>After the reaction has been performed for one cycle, the reaction part stops, and the reaction solution | ||
+ | is thus introduced into the concentrated crystallization part and the work begins. | ||
+ | <br> In this part we mainly use the acrylic material container, aluminum alloy cold source (including | ||
+ | collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor | ||
+ | and semiconductor refrigeration film, etc. | ||
+ | </h4> | ||
+ | |||
+ | <h4> | ||
+ | (图)Fig5 Hontainer made of Acrylics Fig6 Cold source produced by aluminum alloy Fig 7 Reverse osmosis RO membrane Fig9 3D | ||
+ | design drawing | ||
+ | </h4> | ||
+ | |||
+ | |||
+ | <h4>Instructions:Concentrated crystallization part is relatively independent. As long as the system detects | ||
+ | that the internal water level is higher than the reverse osmosis membrane, the part will start to | ||
+ | work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will | ||
+ | be continuously extracted, which continues to improve the concentration of acrylic acid. And the | ||
+ | semiconductor refrigeration film will help keep the solution temperature below the melting point | ||
+ | 286K of acrylic acid. After many rounds of cycles, acrylic acid crystals can be collected on the | ||
+ | bottom cooling source (but mixed with engineered bacteria). | ||
+ | <br> 3)System Controlling | ||
+ | <br> In order to keep as much as possible in line with the actual industrial production, our device involves | ||
+ | a lot of electronic components, pumps, etc., so we designed a set of controlling procedures. | ||
+ | <br> Because of our lack of experience and staff, we chose to master the Arduino controlling board due | ||
+ | to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display, | ||
+ | Relay module, NTC thermistor temperature sensor, Adjustable DC power supply, Semiconductor refrigeration | ||
+ | chip, Arduino controlling panel, Water level sensor, DuPont line, LCD1602 LCD adapter board, Motor | ||
+ | drive extension and so on. | ||
+ | <br> Here is our main program of the controlling system: | ||
+ | </h4> | ||
+ | |||
+ | (压缩文件) | ||
+ | |||
+ | |||
+ | |||
+ | <h3 style="text-align:center">Video</h3> | ||
+ | |||
+ | |||
+ | |||
+ | <h3 style="text-align:center">Impression summary</h3> | ||
+ | |||
+ | <h4>We have no relevant laboratory and company support, staff is also very scarce (the main staff is only | ||
+ | one person). The road of our first HW work was quite stumbling. So in this part, we hope to be able | ||
+ | to provide a lesson that feels most profound: | ||
+ | <br> Before the actual production and debugging, full consideration and deduction must be carried out. | ||
+ | The delivery of the things bought often cost two or three days (in China, we mainly do online shopping, | ||
+ | so the courier time needs to be considered), and the purchase of goods may be inconsistent with what | ||
+ | we wanted. Plus, the negligence of some important details may lead to the purchase of wrong things, | ||
+ | which will waste a lot of valuable time. | ||
+ | <br> If you want to know more or cooperate with us, welcome to contact us | ||
+ | </h4> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <!-- Blog Post Row --> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
</html> | </html> |
Revision as of 10:45, 27 October 2017