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Revision as of 11:12, 27 October 2017
「Interlab」
Introduction
It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by measure the expression level of GFP, in order to help the iGEM community collect data about how reliable will these devices turn out to be in labs around the world.
Provenance and Release
①Individuals responsible for conducting InterLab study
| Individuals | Interlab Part |
|---|---|
| Kangyuan Yu, Haibo Huang, Ziyang Xiao, Shaofeng Liao | created the devices |
| Efan Wang, Long Cheng, HuiPing Shi | conducted the measurements |
| Efan Wang | processed the data |
②Corresponding email
| Individuals | Emails |
|---|---|
| Efan Wang | erfan@hust.edu.cn |
| Kangyuan Yu | 985930862@qq.com |
| Haibo Huang | u201512127@hust.edu.cn |
| Ziyang Xiao | 372657289@qq.com |
| Shaofeng Liao | 15827233830@qq.com |
| HuiPing Shi | 172295915@qq.com |
| Long Cheng | u201512127@hust.edu.cn |
Chassis and Safety
What chassis did you use?
Escherichia coli DH5alpha
What Biosafety Level is your chassis?
BSL1
What PPE did you utilize during your experiments?
Tianming gloves
Songxinjiujiu labcoats
Instrument
What instrument did you use during your measurements?
plate reader
Please provide the brand and model of your instrument.
Flexstation 3
Calibration Protocol
A1. Protocol for Optical Density (OD600) Standard Measurement
Did you use pathlength correction during measurement?
Yes
Number of flashes per well
6
Orbital averaging (mm)
600
What temperature setting did you use during the measurement?
22℃
What type of 96-well plate did you use?
Black plate (preferred)
Did your plate have flat-bottomed or round-bottomed wells?
Flat
A2. Measurement Steps
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B1. Protocol for FIuorescein Fluoresence standard curve
Did you use pathlength correction during measurement?
Yes
Number of flashes per well
6
What gain setting did you use?
Automatic
If you used a filter, what light wavelengths did it pass?
530nm
Emission wavelength
530nm
Excitation wavelength
485nm
Fluorescence reading
Bottom optic
What type of 96-well plate did you use?
Black plate (preferred)
Did your plate have flat-bottomed or round-bottomed wells?
Flat
What temperature setting did you use during the measurement?
22℃
