Difference between revisions of "Team:Arizona State/Notebook"

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<p> This particular PCR ran for 1 hour and 16 minutes. After this, I needed to run a agarose gel to verify if the DNA amplified or not. Once the gel was made, I inputted a kb+1 DNA ladder into the first slot of the gel. I skipped the second slot and proceeded to input 2 µL 6X Loading Dye + 4 µL of PCR into the remaining 8 wells. The gel was ran at 110 V for 45 minutes<p/>  
 
<p> This particular PCR ran for 1 hour and 16 minutes. After this, I needed to run a agarose gel to verify if the DNA amplified or not. Once the gel was made, I inputted a kb+1 DNA ladder into the first slot of the gel. I skipped the second slot and proceeded to input 2 µL 6X Loading Dye + 4 µL of PCR into the remaining 8 wells. The gel was ran at 110 V for 45 minutes<p/>  

Revision as of 21:08, 27 October 2017