Difference between revisions of "Team:TNCR Korea/Part Collection"
| Line 11: | Line 11: | ||
<ul> | <ul> | ||
| − | <li>BBa_K2496000 | + | <li>BBa_K2496000 = FLAG</li> |
<li>BBa_K2496001 = DPP4</li> | <li>BBa_K2496001 = DPP4</li> | ||
<li>BBa_K2496002= strong recombinant </li> | <li>BBa_K2496002= strong recombinant </li> | ||
Revision as of 02:53, 28 October 2017
TNCR_Korea
Part Collection
- BBa_K2496000 = FLAG
- BBa_K2496001 = DPP4
- BBa_K2496002= strong recombinant
- BBa_K2496003= strong anderson promoter(BBa_J23110)
- BBa_K2496004= moderate anderson promoter(BBa_J23111)
- BBa_K2496005= weak anderson promoter(BBa_J23116)
- BBa_K2496006= moderate recombinant
- BBa_K2496007= weak recombinant
Three composite plasmid(strong, moderate, weak) was designed to control the expression of DPP4, because DPP4 can induce inflammation. Strong recombinant plasmid have most strong expression(1) of DPP4 Moderate recombinant plasmid have moderate expression(0.58) of DPP4 Weak recombinant plasmid have most weak expression(0.1) of DPP4
TNCR_Korea constituted a total of three recombinant plasmids. Anderson promoter (strong, moderate, weak.) + FLAG + DPP4. There are two Pst1 sites in the DPP4 sequence, so you can not access the desired 3A assembly method from iGEM. So we used infusion ligation method instead.
