Difference between revisions of "Team:Queens Canada/Basic Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<h3>Best Basic Part Special Prize</h3>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
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<h1><font color="black" face="Arial"><center><span style="font-weight:normal; font-size: 23pt"><br><br><br>
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CsgA-SpyTag Protein</span></center></font></h1><hr/>
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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<p class="big"><font size="5" color="black" face="Corbel">The CsgA-SpyTag Fusion Protein is one half of a split protein system. When SpyTag finds SpyCatcher in solution, they form a covalent bond, joining whatever is fused onto each part of the split protein system. SpyTag is fused onto CsgA (an E. coli biofilm amyloid protein) so that large proteins can be added to the biofilm that are larger than the export limitation of around 60 amino acids.</font></p>
  
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<h1><font color="black" face="Arial"><center><span style="font-weight:normal; font-size: 23pt">Golden Gate Assembly</span></center></font></h1><hr/>
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<p class="big"><font size="5" color="black" face="Corbel">Allows golden gate assembly onto an E. coli biofilm amyloid protein (CsgA) so that a variety of fusion proteins can easily be made. The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate assembly, has its origins in 1996, when for the first time it was shown that multiple inserts could be assembled into a vector backbone using only the sequential or simultaneous activities of a single type IIS restriction enzyme and T4 DNA ligase. Golden Gate Assembly and its derivative methods exploit the ability of Type IIS restriction endonucleases (REases) to cleave DNA outside of the recognition sequence. The inserts and cloning vectors are designed to place the Type IIS recognition site distal to the cleavage site, such that the Type IIS REase can remove the recognition sequence from the assembly. The net result is the ordered and seamless assembly of DNA fragments in one reaction.</font></p>
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<groupparts>iGEM17 Queens_Canada</groupparts>
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Latest revision as of 03:56, 28 October 2017




CsgA-SpyTag Protein



The CsgA-SpyTag Fusion Protein is one half of a split protein system. When SpyTag finds SpyCatcher in solution, they form a covalent bond, joining whatever is fused onto each part of the split protein system. SpyTag is fused onto CsgA (an E. coli biofilm amyloid protein) so that large proteins can be added to the biofilm that are larger than the export limitation of around 60 amino acids.

Golden Gate Assembly



Allows golden gate assembly onto an E. coli biofilm amyloid protein (CsgA) so that a variety of fusion proteins can easily be made. The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate assembly, has its origins in 1996, when for the first time it was shown that multiple inserts could be assembled into a vector backbone using only the sequential or simultaneous activities of a single type IIS restriction enzyme and T4 DNA ligase. Golden Gate Assembly and its derivative methods exploit the ability of Type IIS restriction endonucleases (REases) to cleave DNA outside of the recognition sequence. The inserts and cloning vectors are designed to place the Type IIS recognition site distal to the cleavage site, such that the Type IIS REase can remove the recognition sequence from the assembly. The net result is the ordered and seamless assembly of DNA fragments in one reaction.

<groupparts>iGEM17 Queens_Canada</groupparts>