Difference between revisions of "Team:UCopenhagen/InterLab"
| Line 61: | Line 61: | ||
</div> | </div> | ||
<div class="col-lg-5 col-lg-offset-2 col-sm-6"> | <div class="col-lg-5 col-lg-offset-2 col-sm-6"> | ||
| − | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/b/b9/InterLab-figure_UCopenhagen.png" alt=""> | + | <figure> |
| + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/b/b9/InterLab-figure_UCopenhagen.png" alt="" width="304" height="228"> | ||
| + | |||
| + | <figcaption>Fig1. - Standard curve of fluorescein fluorescence. | ||
| + | Fluorescence in arbitraty units (a.u.), fluorescein concentration in µM.</figcaption> | ||
| + | </figure> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 11:57, 28 October 2017
Introduction
We participated in InterLitab, as we want to be contribute to the scientific progress made through this globe spanning project. In InterLab, 6 test devices are inserted in E.coli D5 α, and the growth and fluorescence is measured.
We used the following plasmids provided by iGEM HQ to transform E.coli:
- Positive control
- Negative control
- Test Device 1: J23101+I13504
- Test Device 2: J23106+I13504
- Test Device 3: J23117+I13504
- Test Device 4: J23101.BCD2.E0040.B0015
- Test Device 5: J23106.BCD2.E0040.B0015
- Test Device 6: J23117.BCD2.E0040.B0015
Calibrations
Before our measurements began, we performed some calibrations: First an OD600 reference point for our plate reader, performed with LUDOX according to the protocol. Here we found a correction factor which can be used to calculate OD from measured absorbance. Our correction fator is 3.11.
Secondly we made a fluorescence standard curve with a serial dilution of fluorescein (figure 1). We used the lower 5 data points to calculate a mean µM fluorescein pr a.u. We chose to use the lower concentration range due to two factors: 1) Linearity is better for the lower fluorescein concentrations, and 2) our measured data has a maximum fluorescence of 500, which makes it more important to have a good fit in the lower range.
