Difference between revisions of "Team:NTHU Taiwan/Contribution"

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1. interlab measurement study
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We have finished the interlab experiments and get lots of results in the page of interlab
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2. Contribution of new parts
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We have contributed new 12 biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Figure 1)
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<h1>Contribution</h1>
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Figure 1. the list of our new 12 parts
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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3.  Validation of composite parts’ function
 
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We constructed three composite parts and all of their function have been validated:
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(1)Part:BBa_K2354010:monobody-GBP HS
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Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it and binds to ER-alpha/EDCs complex.
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(2)Part:BBa_K2354011:INP-ER-alpha HS
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ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
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(3)Part:BBa_K2354012:HRP-his tag
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Horseradish peroxidase is one kind of peroxidase which has heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
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Revision as of 13:23, 28 October 2017

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NTHU_Taiwan

1. interlab measurement study

We have finished the interlab experiments and get lots of results in the page of interlab

2. Contribution of new parts

We have contributed new 12 biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Figure 1)

Figure 1. the list of our new 12 parts

3. Validation of composite parts’ function

We constructed three composite parts and all of their function have been validated:

(1)Part:BBa_K2354010:monobody-GBP HS

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it and binds to ER-alpha/EDCs complex.

(2)Part:BBa_K2354011:INP-ER-alpha HS

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.

(3)Part:BBa_K2354012:HRP-his tag

Horseradish peroxidase is one kind of peroxidase which has heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.