Difference between revisions of "Team:Peking/Notebook"

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                 <h1 style="font-size: xx-large; color: white"><strong>Notebook</strong></h1>
 
                 <h1 style="font-size: xx-large; color: white"><strong>Notebook</strong></h1>
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                    <ul style="margin-left: 0px;">
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            <br>
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            <h2>Week 1</h2>
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            <strong>26/06/2017-02/07/2017</strong><br>
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            Design primers.<br>
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            Construct standard structure of integrase int5 and int8.<br>
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            <h2>Week 2</h2>
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            <strong>03/07/2017-09/07/2017</strong><br>
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            Mutate integrase int8, int10, p22 for Golden Gate Assembly.<br>
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            Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int8, p21, FimB, FimE, HbiF, Vcre, Scre, Cre, Dre, Vika, Lambda-int.<br>
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            Construct the reporter system utilized the structure of attB-Terminator-attP.<br>
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            <h2>Week 3</h2>
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            <strong>10/07/2017-16/07/2017</strong><br>
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            Mutate Bxb1-gp35, phiC31 for Golden Gate Assembly.<br>
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            Ask for some plasmids of repressilator.<br>
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            <h2>Week 4</h2>
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            <strong>17/07/2017-23/07/2017</strong><br>
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            Construct the reporter system of Bxb1, TP901, phiC31.<br>
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            Prepare for repressilator test.<br>
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            <h2>Week 5</h2>
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            <strong>24/07/2017-30/07/2017</strong><br>
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            Construct the expression system of integrase TP901, Bxb1-gp35, phiC31 without RBS.<br>
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            Design Microfluidic device for the oscillation test of repressilator.<br>
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            <h2>Week 6 31/07/2017-06/08/2017</h2>
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            <strong>31/07/2017-06/08/2017</strong><br>
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            Construct the expression system of integrase TP901, Bxb1-gp35 with a series RBS of different transcription initiate rate.<br>
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            Manufacture seven-segment display microfluidic device.<br>
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            <h2>Week 7</h2>
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            <strong>07/08/2017-13/08/2017</strong><br>
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            Construct the expression system of integrase TP901, Bxb1-gp35 with another series RBS of different transcription initiate rate.<br>
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            Image period of LP117 oscillation around 15 hours by microfluidic device.<br>
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            <h2>Week 8</h2>
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            <strong>14/08/2017-20/08/2017</strong><br>
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            Construct the expression system of integrase phiC31 with a series RBS of different transcription initiate rate.<br>
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            Manufacture Sand Clock microfluidic device.<br>
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            Prepare for parts construction.<br>
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            <h2>Week 9</h2>
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            <strong>21/08/2017-27/08/2017</strong><br>
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            Construct the expression system and reporter system of integrase-RDF (recombination directionality factor) fusion protein.<br>
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−
            Test integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
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            Image period of plpt20 oscillation around 7 hours.<br>
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            <h2>Week 1028/08/2017-03/09/2017</h2>
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            <strong>28/08/2017-03/09/2017</strong><br>
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            Make adjustment for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.<br>
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            Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
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            Parts construction of integrase TP901.<br>
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            Site mutation of plpt20 original plasmid.<br>
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            <h2>Week 11</h2>
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            <strong>04/09/2017-10/09/2017</strong><br>
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            Construct the front end of Flip-Flop.<br>
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            Construct the expression system of integrase-RDF (recombination directionality factor) fusion protein.<br>
+
−
            Site mutation of plpt20 original plasmid.<br>
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            <h2>Week 12</h2>
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            <strong>11/09/2017-17/09/2017</strong><br>
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            Make adjustment again for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.<br>
+
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            Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.<br>
+
−
            Construct the reporter system of integrase-RDF (recombination directionality factor) fusion protein.<br>
+
−
            Parts construction of integrase int2, int5.<br>
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            Construct the expression system for oscillator plpt20 with integrase Bxb1-gp35.<br>
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            <h2>Week 13</h2>
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            <strong>18/09/2017-24/09/2017</strong><br>
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            Construct TP901, Bxb1, phiC31 expression system of integrase-RDF.<br>
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            Parts construction of integrase expression system.<br>
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            Construct the expression system for oscillator plpt20 with integrase T7 promoter.<br>
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            <h2>Week 14</h2>
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            <strong>25/09/2017-01/10/2017</strong><br>
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            Test the recombination efficiency of TP901, Bxb1 integrase-RDF by Flow Cytometer.<br>
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            Parts construction of integrase reporter system.<br>
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            <h2>Week 15</h2>
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            <strong>02/10/2017-08/10/2017</strong><br>
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            Make adjustment for integrase-RDF expression system to improve recombination efficiency.<br>
+
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            Test the recombination efficiency of adjusted integrase-RDF.<br>
+
−
            Parts construction of integrase expression system and reporter system.<br>
+
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            Construct reporter system for oscillator plpt41 with T7 promoter, Bxb1-gp35.<br>
+
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            Image repressilator-drived DNA recombination.<br>
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            <h2>Week 16</h2>
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−
            <strong>09/10/2017-15/10/2017</strong><br>
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            <h2>Week 17</h2>
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            <strong>16/10/2017-22/10/2017</strong><br>
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                        <li><a href="#lorem1">Description</a></li>
  
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Revision as of 16:07, 28 October 2017

Peking iGEM 2017

Notebook


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