Difference between revisions of "Team:Hong Kong UCCKE/Design"
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| − | <h3 class="sectiontitle">Mechanism of the Promoter</h3> | + | <h3 class="sectiontitle">Mechanism of the Uric Acid Concentration-Sensitive Promoter</h3> |
<div class="divider"></div> | <div class="divider"></div> | ||
| − | <p style="text-align:left !important;">HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS).</p> | + | <p style="text-align:left !important;">The promoter is designed to be sensitive to the concentration of uric acid. This promoter control the expression of the downstream gene. The promoter session consists of a constitutive promoter J23100, a RBS B0034, a strong repressor KRAB-HucR, a double terminator B0015. HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS).</p> |
| + | <div class="pop"> | ||
| + | <a href="https://static.igem.org/mediawiki/parts/1/13/T--Hong_Kong_UCCKE--Krab.jpg"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/1/13/T--Hong_Kong_UCCKE--Krab.jpg" alt=""> | ||
| + | </a> | ||
| + | <p style="text-align:left !important;">Expression of a strong repressor (mUTS)</p> | ||
| + | |||
| + | <br><p> HucO is an operative site for mUTS to bind. When mUTS is binded to hucO, the expression of downstream gene is restricted according concentration of substrates. The presence of uric acid limits the binding of mUTS to hucO. The limitation varies as the concentration of uric acid.</p> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/a/a0/T--Hong_Kong_UCCKE--hucOgoi.jpg"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/a/a0/T--Hong_Kong_UCCKE--hucOgoi.jpg" alt="" style="width:100%; max-width:500px;"> | ||
| + | </a> | ||
| + | <p style="text-align:left !important;">Operator site and Gene of Interest</p> | ||
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| − | + | <p style="text-align:left !important;">mUTS binds to hucO and GFP is not expressed when uric acid is absent or at very low concentration. Alternatively, the complex detaches from hucO and GFP is expressed according to the concentration of uric acid.</p> | |
| − | + | ||
<div class="pop"> | <div class="pop"> | ||
| − | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2017/3/3a/T--Hong_Kong_UCCKE--genenoua.jpg" data-caption="Image caption"> |
| − | <img src="https://static.igem.org/mediawiki/ | + | <figure><img src="https://static.igem.org/mediawiki/2017/3/3a/T--Hong_Kong_UCCKE--genenoua.jpg" alt=""></figure> |
| − | </ | + | </a> |
| + | <p style="text-align:left !important;">Expression without uric acid (-UA)</p> | ||
</div> | </div> | ||
<div class="pop"> | <div class="pop"> | ||
| − | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2017/3/37/T--Hong_Kong_UCCKE--genewifua.jpg"> |
| − | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/3/37/T--Hong_Kong_UCCKE--genewifua.jpg" alt=""> |
</a> | </a> | ||
| + | <p style="text-align:left !important;">Expression with uric acid (+UA)</p> | ||
</div> | </div> | ||
Revision as of 16:51, 28 October 2017
Mechanism of the Uric Acid Concentration-Sensitive Promoter
The promoter is designed to be sensitive to the concentration of uric acid. This promoter control the expression of the downstream gene. The promoter session consists of a constitutive promoter J23100, a RBS B0034, a strong repressor KRAB-HucR, a double terminator B0015. HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS).
Expression of a strong repressor (mUTS)
HucO is an operative site for mUTS to bind. When mUTS is binded to hucO, the expression of downstream gene is restricted according concentration of substrates. The presence of uric acid limits the binding of mUTS to hucO. The limitation varies as the concentration of uric acid.
Operator site and Gene of Interest
mUTS binds to hucO and GFP is not expressed when uric acid is absent or at very low concentration. Alternatively, the complex detaches from hucO and GFP is expressed according to the concentration of uric acid.
Expression without uric acid (-UA)
Expression with uric acid (+UA)
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