Difference between revisions of "Team:NTHU Taiwan/Composite Part"

Line 2: Line 2:
 
<html>
 
<html>
  
 +
<p>
 +
We constructed three composite parts and all of their function have been validated: (result連結)
 +
</P>
  
 +
<p>
 +
BBa_K2354010
 +
</P>
  
 +
<p>
 +
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.
 +
</P>
  
 +
<p>
 +
BBa_K2354011
 +
</P>
  
−
<div class="column full_size">
+
<p>
−
<h1>Composite Parts</h1>
+
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
−
 
+
</P>
  
 
<p>
 
<p>
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
BBa_K2354012
−
</p>
+
</P>
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
<p>
−
 
+
Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
−
<br>
+
</P>
−
<h3>Best Composite Part Special Prize</h3>
+
−
 
+
−
<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
  
−
<br><br>
 
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
−
<br>
 
−
<div class="highlight">
 
−
<h4>Note</h4>
 
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
 
  
−
</div>
 
  
−
</div>
 
  
 
</html>
 
</html>

Revision as of 16:55, 28 October 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
InterLab
Contribution
Model
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM

NTHU_Taiwan

We constructed three composite parts and all of their function have been validated: (result連結)

BBa_K2354010

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.

BBa_K2354011

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.

BBa_K2354012

Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.