Difference between revisions of "Team:Freiburg/Achievements"
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<h2>Requirements for a Gold Medal</h2> | <h2>Requirements for a Gold Medal</h2> | ||
| − | <h3>Integrated | + | <h3>Integrated human practice</h3> |
<p>The goal of CARTEL™ is to create a safer immune-based therapy. Hearing many concerns about the safety of our project and conducting a survey, we integrated further safety precautions. We successfully tested a construct containing a thymidine kinase which makes T cells sensitive to ganciclovir induced apoptosis. We went over a realistic embedment of our project in the context of acceptance and side effects. Additionally, we evaluated possibilities to optimize the manufacturing process of CAR T cells with the aim to contribute to a therapy, which is affordable and versatile in its use for many cancer patients. Finally, a survey was able to confirm the importance and the need for immunotherapies, which have potential to improve the efficacy and safety of cancer therapies.</p> | <p>The goal of CARTEL™ is to create a safer immune-based therapy. Hearing many concerns about the safety of our project and conducting a survey, we integrated further safety precautions. We successfully tested a construct containing a thymidine kinase which makes T cells sensitive to ganciclovir induced apoptosis. We went over a realistic embedment of our project in the context of acceptance and side effects. Additionally, we evaluated possibilities to optimize the manufacturing process of CAR T cells with the aim to contribute to a therapy, which is affordable and versatile in its use for many cancer patients. Finally, a survey was able to confirm the importance and the need for immunotherapies, which have potential to improve the efficacy and safety of cancer therapies.</p> | ||
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| + | <h3>Improve a previous part or project</h3> | ||
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| + | <h3>Model your project</h3> | ||
| + | <p>Modeling was an important part of our project with which we could determine whenever our understanding of the system has a good substantiation. Combining the hypoxia response element, cAMP response element and CTLA4 promoter in our CARTELTM AND gate to boost the specificity of the CAR expression, was challenging. After modeling different possibilities and comparing the results we designed an AND gate were HIF1A is knocked out and reintroduced under the control of either the cAMP response element promoter or the CTLA4 promoter. By this usage of modeling, we aim to improve the safety and specificity of emerging synthetic CAR therapies.</p> | ||
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| + | <h3>Demonstrate your work</h3> | ||
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Revision as of 18:41, 28 October 2017
Achievements
Most important achievements
Knock Down
We designed...
Kill Switch
Text
Stable Cell lines
Stable cell lines for promoter characterization, knock-down of HIF1A and kill switch were generated via lentiviral transduction and sorted by FACS.
Requirements for a Gold Medal
Integrated human practice
The goal of CARTEL™ is to create a safer immune-based therapy. Hearing many concerns about the safety of our project and conducting a survey, we integrated further safety precautions. We successfully tested a construct containing a thymidine kinase which makes T cells sensitive to ganciclovir induced apoptosis. We went over a realistic embedment of our project in the context of acceptance and side effects. Additionally, we evaluated possibilities to optimize the manufacturing process of CAR T cells with the aim to contribute to a therapy, which is affordable and versatile in its use for many cancer patients. Finally, a survey was able to confirm the importance and the need for immunotherapies, which have potential to improve the efficacy and safety of cancer therapies.
Improve a previous part or project
Model your project
Modeling was an important part of our project with which we could determine whenever our understanding of the system has a good substantiation. Combining the hypoxia response element, cAMP response element and CTLA4 promoter in our CARTELTM AND gate to boost the specificity of the CAR expression, was challenging. After modeling different possibilities and comparing the results we designed an AND gate were HIF1A is knocked out and reintroduced under the control of either the cAMP response element promoter or the CTLA4 promoter. By this usage of modeling, we aim to improve the safety and specificity of emerging synthetic CAR therapies.
Demonstrate your work
Requirements for a Silver Medal
Requirements for a Bronze Medal
