Difference between revisions of "Team:NTHU Taiwan/Composite Part"
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We constructed three composite parts and all of their function have been validated: | We constructed three composite parts and all of their function have been validated: | ||
<ul> | <ul> | ||
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:NTHU_Taiwan/Results">Result</a></li> |
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| − | + | ||
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</ul> | </ul> | ||
</P> | </P> | ||
<p> | <p> | ||
| − | BBa_K2354010 | + | <li><a href="http://parts.igem.org/Part:BBa_K2354010">BBa_K2354010</a></li> |
</P> | </P> | ||
Revision as of 19:41, 28 October 2017
NTHU_Taiwan
We constructed three composite parts and all of their function have been validated:
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.
BBa_K2354011
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
BBa_K2354012
Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
