Difference between revisions of "Team:UNebraska-Lincoln/Design"
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| − | <p>We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test. To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Notebook">lab notebook</a> and <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Results">results</a> sections. Although we made plans to go further with the experimental design, at this point we ran out of time.</p> | + | <p>We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test. Find more information on the Nessler's test on our <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Experiments">Experiments page</a> To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Notebook">lab notebook</a> and <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Results">results</a> sections. Although we made plans to go further with the experimental design, at this point we ran out of time.</p> |
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Revision as of 23:15, 28 October 2017
UNL 2017
Helping reduce methane emissions from livestock
Project Design
We started with our brainstorming and project design stage where we thought through various directions we could take our idea, and then we moved into the experimental design stage where we outlined the things we wanted to accomplish over our project's duration.
Brainstorming Stage
Experimental Stage
We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test. Find more information on the Nessler's test on our Experiments page To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the lab notebook and results sections. Although we made plans to go further with the experimental design, at this point we ran out of time.
Shoulda, Coulda, Woulda
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