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h4 { | h4 { | ||
font-family: 'Karla', sans-serif; | font-family: 'Karla', sans-serif; | ||
+ | color:#4b524a; | ||
} | } | ||
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− | <h4>OBJECTIVE: CREATE | + | <h4>OBJECTIVE: CREATE REPORTER PLASMID</h4> |
<h4>OBJECTIVE: PROMOTER LIBRARY</h4> | <h4>OBJECTIVE: PROMOTER LIBRARY</h4> | ||
<h4>OBJECTIVE: RANDOM LIGATIONS</h4> | <h4>OBJECTIVE: RANDOM LIGATIONS</h4> | ||
− | + | ||
+ | <h4 style="color:#4b524a; font-weight: bold; font-size: 30px;">OBJECTIVE: FREEZE DRYING</h4> | ||
− | <h4>OBJECTIVE: CRISPRi & | + | <h4>OBJECTIVE: CRISPRi & guideRNA EFFICIENCY</h4> |
<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, | <p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, |
Revision as of 18:32, 29 October 2017
RESULTS:
OBJECTIVE: CREATE pgRNA
OBJECTIVE: CREATE REPORTER PLASMID
OBJECTIVE: PROMOTER LIBRARY
OBJECTIVE: RANDOM LIGATIONS
OBJECTIVE: FREEZE DRYING
OBJECTIVE: CRISPRi & guideRNA EFFICIENCY
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.