Line 90: | Line 90: | ||
− | <h4> | + | <h4>STEP 1: Create guideRNA Plasmid</h4> |
Line 99: | Line 99: | ||
− | <h4> | + | <h4>STEP 2: Create Reporter Plasmid </h4> |
− | <h4> | + | <h4>STEP 3 : Create Promoter Library</h4> |
− | + | <h4>STEP 4 : Random Ligations </h4> | |
− | + | <h4>STEP 5: Freeze drying & Revivial</h4> | |
− | + | <h4>STEP 6: CRISPRi & guideRNA efficiency</h4> | |
<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, | <p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, |
Revision as of 18:39, 29 October 2017
RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3 : Create Promoter Library
STEP 4 : Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.