Difference between revisions of "Team:NCKU Tainan/Contribution"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <div class="col">
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      <div id="top">
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      </div>
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      <div id="category" class="vertical-container">
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        <h1 class="wet">Contribution</h1>
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      </div>
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    </div>
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</div>
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<div class="clear"></div>
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<div class="container-fluid">
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    <div id="paragraph" class="paragraph col-md-8 col-md-offset-1 col-xs-offset-1 col-xs-10 os-animation" data-os-animation="fadeIn" data-os-animation-delay="0s">
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      <h2 id="introduction">
 +
        Introduction
 +
      </h2>
 +
      <hr>
 +
      <p>
 +
        Nitrate is toxic to fish or shrimp in water. When nitrate reach a certain concentration level, aquatic animal will start to die. So, in order to maintain good quality of water, concentration of nitrate in water need to be tested every day. In corporate
 +
        to our team’s project “NO PROBLEM” this year, decide to sense the concentration of nitrite and nitrate as a part of our project.
 +
      </p>
 +
      <h2 id="description">
 +
        Description
 +
      </h2>
 +
      <hr>
 +
      <p>
 +
        Part: BBa_K381001 was first designed by Katharine Coyte from team BCCS-Bristol in iGEM 2010. It is a Nitrate reporter, PyeaR - GFP composite. The team BCCS-Bristol only test the BioBrick’s sensitivity at a higher concentration, but the Nitrate level of
 +
        aquatic water usually won’t reach 1 mM. Therefore, we carry out experiment by testing the fluorescence intensity with ppm level of Potassium Nitrate, which is much lower than all the teams before. The results are shown below.
 +
      </p>
 +
      <div class="row imagerow">
 +
        <div class="col-12">
 +
          <img src="https://static.igem.org/mediawiki/2017/4/4d/T--NCKU_Tainan--contribution1.png" alt="contribution image 1" class="img-responsive">
 +
          <p>
 +
            Figure 1. The fluorescence intensity of different Nitrate concentration taken the logarithm at 8, 12 and 24 hours.
 +
          </p>
 +
        </div>
 +
      </div>
 +
      <p>
 +
        It shows that the BioBrick BBa_K381001 have nice sensitivity not just in high Nitrate concentration, but low as well. Many teams had done improvements of K381001, but our team bring out the most beautiful data. And we also take X axis logarithm, which
 +
        can make our data become linear. Not only easier to read, but also more meaningful.
 +
      </p>
 +
      <p>
 +
        The data is shown as below.
 +
      </p>
 +
      <table class="table">
 +
        <thead>
 +
          <tr>
 +
            <th>
 +
              ppm conc
 +
            </th>
 +
            <th>
 +
              8 hours
 +
            </th>
 +
            <th>
 +
              12 hours
 +
            </th>
 +
            <th>
 +
              24 hours
 +
            </th>
 +
          </tr>
 +
        </thead>
 +
        <tbody>
 +
          <tr>
 +
            <td>
 +
              0
 +
            </td>
 +
            <td>
 +
              454.623
 +
            </td>
 +
            <td>
 +
              546.841
 +
            </td>
 +
            <td>
 +
              620.402
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              2
 +
            </td>
 +
            <td>
 +
              689.961
 +
            </td>
 +
            <td>
 +
              754.085
 +
            </td>
 +
            <td>
 +
              821.113
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              4
 +
            </td>
 +
            <td>
 +
              766.835
 +
            </td>
 +
            <td>
 +
              866.846
 +
            </td>
 +
            <td>
 +
              922.813
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              6
 +
            </td>
 +
            <td>
 +
              816.472
 +
            </td>
 +
            <td>
 +
              916.119
 +
            </td>
 +
            <td>
 +
              972.653
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              8
 +
            </td>
 +
            <td>
 +
              831.843
 +
            </td>
 +
            <td>
 +
              931.43
 +
            </td>
 +
            <td>
 +
              985.14
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              10
 +
            </td>
 +
            <td>
 +
              900.114
 +
            </td>
 +
            <td>
 +
              998.935
 +
            </td>
 +
            <td>
 +
              1058.844
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              20
 +
            </td>
 +
            <td>
 +
              962.493
 +
            </td>
 +
            <td>
 +
              1093.076
 +
            </td>
 +
            <td>
 +
              1148.216
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              40
 +
            </td>
 +
            <td>
 +
              1090.926
 +
            </td>
 +
            <td>
 +
              1200.976
 +
            </td>
 +
            <td>
 +
              1252.106
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              60
 +
            </td>
 +
            <td>
 +
              1129.622
 +
            </td>
 +
            <td>
 +
              1253.052
 +
            </td>
 +
            <td>
 +
              1295.002
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              80
 +
            </td>
 +
            <td>
 +
              1240.331
 +
            </td>
 +
            <td>
 +
              1341.711
 +
            </td>
 +
            <td>
 +
              1373.601
 +
            </td>
 +
          </tr>
 +
          <tr>
 +
            <td>
 +
              100
 +
            </td>
 +
            <td>
 +
              1259.766
 +
            </td>
 +
            <td>
 +
              1385.946
 +
            </td>
 +
            <td>
 +
              1449.946
 +
            </td>
 +
          </tr>
 +
        </tbody>
 +
      </table>
 +
    </div>
 +
    <div id="sidemenu" class="col-md-2">
 +
      <div class="list-group">
 +
        <a onclick="scrollto('#introduction')" class="list-group-item">Introduction</a>
 +
        <hr>
 +
        <a onclick="scrollto('#description')" class="list-group-item">Description</a>
 +
        <hr>
 +
        <a class="list-group-item top"><i  onclick="scrollto('#top')" class="fa fa-arrow-up fa-1x" aria-hidden="true"></i></a>
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      </div>
 +
    </div>
  
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<div class="column full_size">
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<h1>Contribution</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
+
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<br><br>
+
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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</p>
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</div>
 
</div>
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Revision as of 01:45, 30 October 2017

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JUDGING FORM

NCKU_Tainan

Contribution

Introduction

Nitrate is toxic to fish or shrimp in water. When nitrate reach a certain concentration level, aquatic animal will start to die. So, in order to maintain good quality of water, concentration of nitrate in water need to be tested every day. In corporate to our team’s project “NO PROBLEM” this year, decide to sense the concentration of nitrite and nitrate as a part of our project.

Description

Part: BBa_K381001 was first designed by Katharine Coyte from team BCCS-Bristol in iGEM 2010. It is a Nitrate reporter, PyeaR - GFP composite. The team BCCS-Bristol only test the BioBrick’s sensitivity at a higher concentration, but the Nitrate level of aquatic water usually won’t reach 1 mM. Therefore, we carry out experiment by testing the fluorescence intensity with ppm level of Potassium Nitrate, which is much lower than all the teams before. The results are shown below.

contribution image 1

Figure 1. The fluorescence intensity of different Nitrate concentration taken the logarithm at 8, 12 and 24 hours.

It shows that the BioBrick BBa_K381001 have nice sensitivity not just in high Nitrate concentration, but low as well. Many teams had done improvements of K381001, but our team bring out the most beautiful data. And we also take X axis logarithm, which can make our data become linear. Not only easier to read, but also more meaningful.

The data is shown as below.

ppm conc 8 hours 12 hours 24 hours
0 454.623 546.841 620.402
2 689.961 754.085 821.113
4 766.835 866.846 922.813
6 816.472 916.119 972.653
8 831.843 931.43 985.14
10 900.114 998.935 1058.844
20 962.493 1093.076 1148.216
40 1090.926 1200.976 1252.106
60 1129.622 1253.052 1295.002
80 1240.331 1341.711 1373.601
100 1259.766 1385.946 1449.946