Difference between revisions of "Team:NCKU Tainan/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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          <h1 class="wet">Safety</h1>
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    <div id="paragraph" class="paragraph col-md-8 col-md-offset-1 col-xs-offset-1 col-xs-10">
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<h5>Safe Project Design</h5>
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      <h2 id="safelaboratory">
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        Safe Laboratory
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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      </h2>
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      <hr>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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        <div class="col-md-6">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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          <img src="https://static.igem.org/mediawiki/2017/2/2e/T--NCKU_Tainan--safety1.jpg" alt="safety image 1" class="img-responsive">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        </div>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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        <div class="col-md-6">
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</ul>
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          <img src="https://static.igem.org/mediawiki/2017/0/00/T--NCKU_Tainan--safety2.jpg" alt="safety image 2" class="img-responsive">
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        </div>
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      </div>
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      <p>
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        Before stepping into the laboratory, all the members in our team must underwent training for laboratory safety. The main topics of the training program includes:
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      </p>
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      <div class="padding_left">
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        <ol>
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          <li> Laboratory & Personal Protective Equipment</li>
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          <br>
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          <li>Operation Procedure for Handling biological material</li>
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          <br>
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          <li>Operation Procedure for Handling Toxic Chemicals</li>
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          <br>
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          <li>Operation Procedure for Emergency Situation</li>
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        </ol>
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      </div>
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      <p class="margin_bottom">
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        Our training program was based on Safety Guidelines for <a href="http://www.cdc.gov.tw/downloadfile.aspx?fid=F3317C2068336173">Biosafety Level 1 to Level 3</a> released by Centers for Disease Control (CDC).
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      </p>
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      <h2 id="safedesign">
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        Safe Design
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      </h2>
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      <hr>
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      <h3>
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        A.&nbsp;&nbsp;Chassis Organism
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      </h3>
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      <p>
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        We us two strains of Escherichia coli as chassis organism for our Project, one is “E. coli str. K-12. MG1655” and the other is “E. coli DH5a”. Both of them are not pathogen for human beings therefor is totally safe and is handled under guideline for biosafety.
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      </p>
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      <h3>
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        B.&nbsp;&nbsp;New Part
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      </h3>
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      <p>
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        In our project, we introduce three new protein for our nitrate regulation pathway: Nitrite reductase (BBa_K2275001), Glutamate dehydrogenase (BBa_K2275002), and Glutamine synthetase (BBa_K2275003). They were originally expressed by E. coli K-12, Pseudomonas putida, and Pseudomonas aeruginosa PAO1, all of them are safety level 1 organism and therefore being harmless to human beings.
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      </p>
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      <h3>
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        C.&nbsp;&nbsp;Device
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      </h3>
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      <ol class="margin_bottom">
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        <li>Sensing Boat</li>
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        <p>
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          <br>
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          The sensing well (in which we settle sensing E. coli) in our boat is designed to be sealed and disposable, and our boat is waterproof (of course), so the E. coli will never escape from our sensing boat as well as the user reclaim them properly.
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        </p>
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        <li>Regulation Box</li>
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        <p>
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          <br>
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          We use E. coli with three construction (nitrate reductase, glutamate dehydrogenase, glutamine) for our nitrate regulation purpose. In order to prevent our regulation E. coli from escaping, we put a filter on both side of the regulation box, and also us a UV light for sterilization before we dispose the water our of our box.
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        </p>
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      </ol>
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      <h2 id="biosafetytest">
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        Biosafety Test
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      </h2>
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      <hr>
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      <p>
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        To proof that the filter and UV light work successfully, we conduct a simple experiment. First, we put some E. coli into our device, settle the filter and UV light at the right place, and then turn on our device. We took some water sample from the exit of our device, which supposed to be discharge into the fish pond in our project, and then apply the water sample on the agar plate. The number of the patch showed on the plate will tell us how efficient our safety design is.
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      </p>
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      <div class="row imagerow">
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        <div class="col-md-6">
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          <img src="https://static.igem.org/mediawiki/2017/6/68/T--NCKU_Tainan--safety3.jpg" alt="safety image 3" class="img-responsive">
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        </div>
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        <div class="col-md-6">
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          <img src="https://static.igem.org/mediawiki/2017/4/43/T--NCKU_Tainan--safety4.jpg" alt="safety image 4" class="img-responsive">
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        </div>
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      </div>
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      <p>
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        Twelve hours later, the plate was totally clean and no bacteria patch showed on the it. On the other hand, there a lot of patches appear on the control plate (with no filter nor UV light). This result suggest that our biosafety design works successfully and has the ability to prevent our E. coli from escaping.
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        <a onclick="scrollto('#safelaboratory')" class="list-group-item">Safe Laboratory</a>
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        <hr>
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        <a onclick="scrollto('#safedesign')" class="list-group-item">Safe Design</a>
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        <hr>
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        <a onclick="scrollto('#biosafetytest')" class="list-group-item">Biosafety Test</a>
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        <hr>
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Revision as of 01:53, 30 October 2017

Safety

Safe Laboratory

safety image 1
safety image 2

Before stepping into the laboratory, all the members in our team must underwent training for laboratory safety. The main topics of the training program includes:

  1. Laboratory & Personal Protective Equipment

  2. Operation Procedure for Handling biological material

  3. Operation Procedure for Handling Toxic Chemicals

  4. Operation Procedure for Emergency Situation

Our training program was based on Safety Guidelines for Biosafety Level 1 to Level 3 released by Centers for Disease Control (CDC).

Safe Design

A.  Chassis Organism

We us two strains of Escherichia coli as chassis organism for our Project, one is “E. coli str. K-12. MG1655” and the other is “E. coli DH5a”. Both of them are not pathogen for human beings therefor is totally safe and is handled under guideline for biosafety.

B.  New Part

In our project, we introduce three new protein for our nitrate regulation pathway: Nitrite reductase (BBa_K2275001), Glutamate dehydrogenase (BBa_K2275002), and Glutamine synthetase (BBa_K2275003). They were originally expressed by E. coli K-12, Pseudomonas putida, and Pseudomonas aeruginosa PAO1, all of them are safety level 1 organism and therefore being harmless to human beings.

C.  Device

  1. Sensing Boat


    2. The sensing well (in which we settle sensing E. coli) in our boat is designed to be sealed and disposable, and our boat is waterproof (of course), so the E. coli will never escape from our sensing boat as well as the user reclaim them properly.

  2. Regulation Box


    3. We use E. coli with three construction (nitrate reductase, glutamate dehydrogenase, glutamine) for our nitrate regulation purpose. In order to prevent our regulation E. coli from escaping, we put a filter on both side of the regulation box, and also us a UV light for sterilization before we dispose the water our of our box.

Biosafety Test

To proof that the filter and UV light work successfully, we conduct a simple experiment. First, we put some E. coli into our device, settle the filter and UV light at the right place, and then turn on our device. We took some water sample from the exit of our device, which supposed to be discharge into the fish pond in our project, and then apply the water sample on the agar plate. The number of the patch showed on the plate will tell us how efficient our safety design is.

safety image 3
safety image 4

Twelve hours later, the plate was totally clean and no bacteria patch showed on the it. On the other hand, there a lot of patches appear on the control plate (with no filter nor UV light). This result suggest that our biosafety design works successfully and has the ability to prevent our E. coli from escaping.