Difference between revisions of "Team:NYU Abu Dhabi/Demonstrate"
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<video width="660" height="450" controls> | <video width="660" height="450" controls> | ||
| − | <source src="https://static.igem.org/mediawiki/2017/ | + | <source src="https://static.igem.org/mediawiki/2017/2/26/Demovideo_final.mp4"> |
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<li> In the wells labelled "+" and "-", add the solution from the tube labelled "C" using one of the pipettes. Make sure to add enough volume to fill each well. | <li> In the wells labelled "+" and "-", add the solution from the tube labelled "C" using one of the pipettes. Make sure to add enough volume to fill each well. | ||
<li> Taking the clean pipette, add your solution from the tube labelled "S" to the remaining wells. | <li> Taking the clean pipette, add your solution from the tube labelled "S" to the remaining wells. | ||
| + | <li> Seal the top of the PDMS plate using the provided optical adhesive. | ||
<li> Insert the lid back onto the device and wait for 20 minutes. | <li> Insert the lid back onto the device and wait for 20 minutes. | ||
<li> After 20 minutes, you results are ready for visualization. | <li> After 20 minutes, you results are ready for visualization. | ||
Revision as of 15:48, 30 October 2017
The provided kit contains all you need for rapid detection of shiga toxin-producing E. coli. Inside you'll find:
- A cotton swab
- 2 disposable Pasteur Pipettes
- 2 solutions labelled "S" (for sample) and "C" (for control)
- A PDMS chip
- A small sheet of optical adhesive
To use the device simply follow the remaining steps:
- Insert the USB into any portable battery pack or into a USB-to-wall adaptor to begin warming the device.
- Swab your food sample using the provided cotton swab and swirl it into the solution labelled "S".
- Place the provided PDMS chip with the wells open towards you.
- In the wells labelled "+" and "-", add the solution from the tube labelled "C" using one of the pipettes. Make sure to add enough volume to fill each well.
- Taking the clean pipette, add your solution from the tube labelled "S" to the remaining wells.
- Seal the top of the PDMS plate using the provided optical adhesive.
- Insert the lid back onto the device and wait for 20 minutes.
- After 20 minutes, you results are ready for visualization.
If your samples are brighter than the well marked "-", your samples may be contaminated by Shiga toxin-producing E. coli and we suggest discarding them.
