Difference between revisions of "Team:Gaston Day School/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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    <h1>Project Description</h1>
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<h5> Special track teams </h5>
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    <h2>Overview</h2>
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      <p>This year, our team is working on biofuel production using <i>E. coli</i>. In the US, most biofuel production comes from either corn or sugar cane. Both of these crops require arable land and pull resources from the food supply. Algae is another option, but it requires land area for growing ponds. <i>E. coli</i>, on the other hand, can be grown in fermenters in a factory and do not affect the food supply or remove arable land from other productive use. <i>E. coli</i> naturally produces several alcohols that can be used as biofuels, including isopropanol, isobutanol, ethanol, and sec-butanol. Unfortunately, <i>E. coli</i> also has pathways that break down these alcohols when the concentration increases to prevent toxic levels from occurring. The combination of increasing the natural resistance to these alcohols as well as upregulating the production of one or more of these alcohols could make this production methods commercially viable.</p>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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    <h2><i>E. coli</i></h2>
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    <p>Escherichia coli (<i>E. coli</i>) bacteria normally live in the intestines of people and animals. Most <i>E. coli</i> are harmless and actually are an important part of a healthy human intestinal tract. However, some <i>E. coli</i> are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of <i>E. coli</i> that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons.</p>
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    <p><i>E. coli</i> consists of a diverse group of bacteria. Pathogenic <i>E. coli</i> strains are categorized into pathotypes. Six pathotypes are associated with diarrhea and collectively are referred to as diarrheagenic <i>E. coli</i>.</p>
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        <li>Shiga toxin-producing <i>E. coli</i> (STEC)—STEC may also be referred to as Verocytotoxin-producing <i>E. coli</i> (VTEC) or enterohemorrhagic <i>E. coli</i> (EHEC). This pathotype is the one most commonly heard about in the news in association with foodborne outbreaks.</li>
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        <li>Enterotoxigenic <i>E. coli</i> (ETEC)</li>
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        <li>Enteropathogenic <i>E. coli</i> (EPEC)</li>
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        <li>Enteroaggregative <i>E. coli</i> (EAEC)</li>
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        <li>Enteroinvasive <i>E. coli</i> (EIEC)</li>
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        <li>Diffusely adherent <i>E. coli</i> (DAEC)</li>
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    <h2>Results</h2>
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    <h2>Conclusion</h2>
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    <h2>Future Development</h2>
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        <li>General Information. (2015, November 06). Retrieved October 20, 2017, from <a href="https://www.cdc.gov/ecoli/general/index.html" >https://www.cdc.gov/ecoli/general/index.html</a></li>
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Revision as of 18:42, 30 October 2017

Project Description

Overview

This year, our team is working on biofuel production using E. coli. In the US, most biofuel production comes from either corn or sugar cane. Both of these crops require arable land and pull resources from the food supply. Algae is another option, but it requires land area for growing ponds. E. coli, on the other hand, can be grown in fermenters in a factory and do not affect the food supply or remove arable land from other productive use. E. coli naturally produces several alcohols that can be used as biofuels, including isopropanol, isobutanol, ethanol, and sec-butanol. Unfortunately, E. coli also has pathways that break down these alcohols when the concentration increases to prevent toxic levels from occurring. The combination of increasing the natural resistance to these alcohols as well as upregulating the production of one or more of these alcohols could make this production methods commercially viable.

E. coli

 

Escherichia coli (E. coli) bacteria normally live in the intestines of people and animals. Most E. coli are harmless and actually are an important part of a healthy human intestinal tract. However, some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons.

E. coli consists of a diverse group of bacteria. Pathogenic E. coli strains are categorized into pathotypes. Six pathotypes are associated with diarrhea and collectively are referred to as diarrheagenic E. coli.

  • Shiga toxin-producing E. coli (STEC)—STEC may also be referred to as Verocytotoxin-producing E. coli (VTEC) or enterohemorrhagic E. coli (EHEC). This pathotype is the one most commonly heard about in the news in association with foodborne outbreaks.
  • Enterotoxigenic E. coli (ETEC)
  • Enteropathogenic E. coli (EPEC)
  • Enteroaggregative E. coli (EAEC)
  • Enteroinvasive E. coli (EIEC)
  • Diffusely adherent E. coli (DAEC)

Results

Conclusion

Future Development

References

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056