Difference between revisions of "Team:NTHU Taiwan/Composite Part"

Line 1: Line 1:
 +
{{:Team:NTHU_Taiwan/MenuBar}}
 
<html>
 
<html>
  
Line 10: Line 11:
 
#bodyContent {background-color:#f6f6e3;}
 
#bodyContent {background-color:#f6f6e3;}
 
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
/**************************************************************** MENU ***************************************************************/
 
/* Wrapper for the menu */
 
 
.igem_2017_menu_wrapper {
 
border-bottom: 3px solid #c2dac0;
 
background-color:#f6f6e3;
 
font-family:'Open Sans', sans-serif;
 
overflow: hidden;
 
}
 
 
.igem_2017_menu_wrapper a { 
 
float: left;
 
font-size: 1.2em;
 
color: #6c5070;
 
text-align: center;
 
padding: 25px 20px;
 
text-decoration: none;
 
}
 
 
.munu_img img{
 
position: fixed;
 
height: 9%;
 
}
 
 
/* this hides the scrollbar to keep view consistency */
 
.igem_2017_menu_wrappe::-webkit-scrollbar {
 
display: none;
 
}
 
 
.submenu_wrapper {
 
float: left;
 
  overflow: hidden;
 
}
 
 
.submenu_wrapper .menu_button {
 
letter-spacing: 1px;
 
text-transform: uppercase;
 
font-size: 1.2em;
 
border: none;
 
outline: none;
 
color: #6c5070;
 
padding: 25px 20px;
 
background-color: inherit;
 
}
 
 
.igem_2017_menu_wrapper a:hover, .submenu_wrapper:hover .menu_button {
 
color: #DF6A6A;
 
}
 
 
.submenu_button {
 
display: none;
 
position: absolute;
 
background-color: rgba(246, 246, 227, 0.7);
 
min-width: 160px;
 
z-index: 1;
 
}
 
 
.submenu_button a {
 
float: none;
 
color: #6c5070;
 
padding: 8px 16px;
 
text-decoration: none;
 
display: block;
 
text-align: left;
 
}
 
 
.submenu_button a:hover {
 
font-size: 1.3em;
 
 
}
 
 
.submenu_wrapper:hover .submenu_button {
 
display: block;
 
}
 
 
/*menu_wrapper left space*/
 
.igem_2017_menu_wrapper .menu_right_margin {
 
margin-left: 160px;
 
}
 
 
/*menu_wrapper right space*/
 
.igem_2017_menu_wrapper .menu_bottom_padding {
 
width: 100%;
 
height: 30px;
 
float:left;
 
}
 
 
/* styling for the menu button when it is the current page */
 
.current_page {
 
color:#df6a6a !important;
 
}
 
 
/* when the page size is bigger than 800px, this show/hide control is hidden by default */
 
.igem_2017_menu_wrapper #display_menu_control {
 
display:none;
 
text-align:center;
 
}
 
 
 
  
Line 136: Line 39:
  
 
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
 
.igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
padding: 40px 0px 15px 0px;  
+
padding: 50px 0px 15px 0px;  
 
border-bottom: 0px;  
 
border-bottom: 0px;  
 
color: #2C2C2C;
 
color: #2C2C2C;
Line 143: Line 46:
 
}
 
}
  
#pdf {
+
.igem_2017_content_wrapper .content {
 
width: 950px;
 
width: 950px;
height: 1200px;
 
 
margin: 2em auto;
 
margin: 2em auto;
padding-top: 20px;
 
 
}
 
}
  
#pdf object {
+
.igem_2017_content_wrapper .content p{
 +
color: #2C2C2C;
 +
font-size: 1.4em;
 +
line-height: 1.3em;
 +
padding: 0px 0px;
 +
text-align: justify;
 +
text-justify:inter-ideograph;
 +
}
 +
 
 +
.igem_2017_content_wrapper .content img{
 +
margin: auto;
 
display: block;
 
display: block;
border: solid 2px #2c2c2c;
+
float: center;
 +
padding: 20px 0px 30px 0px;
 
}
 
}
  
/***************************************************** RESPONSIVE STYLING ****************************************************/
+
.column .full_size {
 +
width: 100%;
 +
padding-top: 50px;
 +
}
  
/* IF THE SCREEN IS LESS THAN 1200PX */
+
/* class for a half width column */
@media only screen and (max-width: 1200px) {
+
.column .half_size {
 +
width: 50%;
 +
float: left;
 +
}
  
#content {width:100%; }
+
#pdf {
.igem_2017_menu_wrapper {width:100%; right:0;}
+
width: 950px;
.highlight {padding:10px 0px;}
+
height: 1200px;
.igem_2017_menu_wrapper #display_menu_control { display:none; }
+
margin: 2em auto;
#menu_content { display:block;}
+
padding-top: 20px;
.menu_button.direct_to_page {padding-left: 17px;}
+
+
 
}
 
}
  
/* IF THE SCREEN IS LESS THAN 800PX */
+
#pdf object {
@media only screen and (max-width: 800px) {
+
display: block;
 
+
border: solid 2px #2c2c2c;
.igem_2017_menu_wrapper { width:100%; height: 15%; position:relative; left:0%;}
+
.igem_2017_content_wrapper {width:100%; margin-left:0px;}
+
.column.half_size  {width:100%; }
+
.column.full_size img, .column.half_size img {  width: 100%; padding: 10px 0px;}
+
.highlight {padding:15px 5px;}
+
.igem_2017_menu_wrapper #display_menu_control { display:block; }
+
#menu_content { display:none;}
+
.igem_2017_menu_wrapper .menu_button .expand_collapse_icon { width: 5%; }
+
.menu_bottom_padding {display:none;}
+
.menu_button.direct_to_page { padding-left: 36px; }
+
 
}
 
}
 
 
</style>
 
</style>
  
Line 194: Line 99:
 
</head>
 
</head>
  
<!-- <div class="munu_img">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan">
 
<img src="https://static.igem.org/mediawiki/2017/1/1f/T--NTHU_Taiwan--HOME--logo.png">
 
</a>
 
</div> -->
 
 
<div class="igem_2017_menu_wrapper" >
 
<!-- this div is hidden by default and will only be displayed if the screen size is too small -->
 
<div class="menu_button" id="display_menu_control">
 
MENU 
 
</div>
 
 
 
<div id="menu_content">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan">
 
<div class="menu_button menu_right_margin">
 
HOME
 
</div>
 
</a>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">TEAM</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Team">
 
<div id="Team_page">
 
Team
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Attributions">
 
<div  id="Attributions_page">
 
Attributions
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Collaborations">
 
<div  id="Collaborations_page">
 
Collaborations
 
</div>
 
</a>
 
</div>
 
</div>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">PROJECT</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Description">
 
<div id="Description_page">
 
Description
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Design">
 
<div  id="Design_page">
 
Design
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Contribution">
 
<div  id="Contribution_page">
 
Contribution
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Model">
 
<div  id="Model_page">
 
Model
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Results">
 
<div  id="Results_page">
 
Results
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Demonstrate">
 
<div  id="Demonstrate_page">
 
Demonstrate
 
</div>
 
</a>
 
 
</div>
 
</div>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">WET LAB</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Experiments">
 
<div  id="Experiments_page">
 
Experiments
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Protocol">
 
<div  id="Protocol_page">
 
Protocol
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Notebook">
 
<div  id="Notebook_page">
 
Notebook
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/InterLab">
 
<div  id="InterLab_page">
 
InterLab
 
</div>
 
</a>
 
</div>
 
</div>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">PARTS</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Basic_Part">
 
<div  id="Basic_Part_page">
 
Basic Parts
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Composite_Part">
 
<div  id="Composite_Part_page">
 
Composite Parts
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Part_Collection">
 
<div  id="Part_Collection_page">
 
Part Collection
 
</div>
 
</a>
 
</div>
 
</div>
 
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Safety">
 
<div class="menu_button">
 
SAFETY
 
</div>
 
</a>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">HUMAN PRACTICES</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/HP/Silver">
 
<div id="Silver_page">
 
Silver HP
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/HP/Gold_Integrated">
 
<div  id="Gold_Integrated_page">
 
Integrated and Gold
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Engagement">
 
<div  id="Engagement_page">
 
Public Engagement
 
</div>
 
</a>
 
</div>
 
</div>
 
 
<div class="submenu_wrapper">
 
<button class="menu_button">AWARDS</button>
 
<div class="submenu_button">
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Applied_Design">
 
<div id="Applied_Design_page">
 
Applied Design
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Entrepreneurship">
 
<div  id="Entrepreneurship_page">
 
Integrated Human Practices
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Hardware">
 
<div  id="Hardware_page">
 
Hardware
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Model">
 
<div  id="Model_page">
 
Model
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Software">
 
<div  id="Software_page">
 
Software
 
</div>
 
</a>
 
<a href="https://2017.igem.org/Team:NTHU_Taiwan/Criteria">
 
<div  id="Criteria_page">
 
Criteria
 
</div>
 
</a>
 
 
</div>
 
</div>
 
 
<a href="https://igem.org/2017_Judging_Form?team=NTHU_Taiwan">
 
<div class="menu_button">
 
JUDGING FORM
 
</div>
 
</a>
 
 
</div>
 
 
</div>
 
  
  
Line 397: Line 104:
 
<!-- start of content -->
 
<!-- start of content -->
 
<div class="igem_2017_content_wrapper">
 
<div class="igem_2017_content_wrapper">
<img width="25%" src="https://static.igem.org/mediawiki/2017/e/e2/T--NTHU_Taiwan--Notebook--Top.png">
 
 
<div style="text-align: center">
 
<div style="text-align: center">
 
<h1 style="color:#DF6A6A">Composite Parts
 
<h1 style="color:#DF6A6A">Composite Parts
Line 404: Line 110:
 
<hr width="20%" />
 
<hr width="20%" />
 
</div>
 
</div>
 +
<div class="content">
  
 +
<br>
 +
<p>
 +
We constructed three composite parts and all of their function have been validated:<a href="https://2017.igem.org/Team:NTHU_Taiwan/Results">Results</a></td>
 +
</P>
  
<style>
 
p{
 
width:1000px;
 
font-size:20px;
 
text-align: justify;
 
text-justify:inter-ideograph;
 
line-height: 30px;
 
}
 
</style>
 
<body>
 
<center>
 
  
 +
<table style="border:3px #cccccc solid;" cellpadding="10" border='1';"font-size:18px";"font-family:Comic Sans MS">
 +
 <tr>
 +
 <td style="background-color:#99c2ff">biobrick</td>
 +
 <td style="background-color:#99c2ff">type</td>
 +
  <td style="background-color:#99c2ff">name</td>
 +
  <td style="background-color:#99c2ff">description</td>
 +
  <td style="background-color:#99c2ff">designer</td>
 +
  <td style="background-color:#99c2ff">length</td>
 +
 </tr>
 +
 <tr>
 +
  <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354010">K2354010</a></td>
 +
 <td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">monobody-GBP HS</td>
 +
  <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface.</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">850</td>
 +
 </tr>
 +
 <tr>
 +
 <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354011">K2354011</a></td>
 +
 <td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">INP-ER-alpha HS </td>
 +
  <td style="background-color:#ffe680">Expression of ER-alpha on the membrane of <I>E. coli</I> and ER-alpha can bind to EDCs</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">2284</td>
 +
 </tr>
 +
 <tr>
 +
 <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354012">K2354012</a></td>
 +
 <td style="background-color:#ffe680">composite</td>
 +
  <td style="background-color:#ffe680">HRP-his tag</td>
 +
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
 +
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
 +
  <td style="background-color:#ffe680">1325</td>
 +
 </tr>
 +
</table>
  
  
<p>
+
<p>
We constructed three composite parts and all of their function have been validated:<a href="https://2017.igem.org/Team:NTHU_Taiwan/Results">Results</a></td>
+
<a href="http://parts.igem.org/Part:BBa_K2354010">K2354010</a></td>
</P>
+
</P>
<br>
+
  
<table style="border:3px #cccccc solid;" cellpadding="10" border='1';"font-size:18px";"font-family:Comic Sans MS">
+
<p>
 <tr>
+
<img src="https://static.igem.org/mediawiki/2017/7/71/T--NTHU_Taiwan--Parts--10.png">
 <td style="background-color:#99c2ff">biobrick</td>
+
</p>
 <td style="background-color:#99c2ff">type</td>
+
  <td style="background-color:#99c2ff">name</td>
+
  <td style="background-color:#99c2ff">description</td>
+
  <td style="background-color:#99c2ff">designer</td>
+
  <td style="background-color:#99c2ff">length</td>
+
 </tr>
+
 <tr>
+
  <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354010">K2354010</a></td>
+
 <td style="background-color:#ffe680">composite</td>
+
  <td style="background-color:#ffe680">monobody-GBP HS</td>
+
  <td style="background-color:#ffe680">Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface.</td>
+
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
+
  <td style="background-color:#ffe680">850</td>
+
 </tr>
+
 <tr>
+
 <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354011">K2354011</a></td>
+
 <td style="background-color:#ffe680">composite</td>
+
  <td style="background-color:#ffe680">INP-ER-alpha HS </td>
+
  <td style="background-color:#ffe680">Expression of ER-alpha on the membrane of <I>E. coli</I> and ER-alpha can bind to EDCs</td>
+
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
+
  <td style="background-color:#ffe680">2284</td>
+
 </tr>
+
 <tr>
+
 <td style="background-color:#ffe680"><a href="http://parts.igem.org/Part:BBa_K2354012">K2354012</a></td>
+
 <td style="background-color:#ffe680">composite</td>
+
  <td style="background-color:#ffe680">HRP-his tag</td>
+
  <td style="background-color:#ffe680">Degrade Estrogen Disrupt Chemicals</td>
+
  <td style="background-color:#ffe680">Chan, Wei-Jen</td>
+
  <td style="background-color:#ffe680">1325</td>
+
 </tr>
+
</table>
+
<br>
+
  
<p>
 
<a href="http://parts.igem.org/Part:BBa_K2354010">K2354010</a></td>
 
</P>
 
  
<p>
 
<img src="https://static.igem.org/mediawiki/2017/7/71/T--NTHU_Taiwan--Parts--10.png">
 
</p>
 
<br>
 
  
  
 +
<p>
 +
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.
 +
</P>
  
<p>
 
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.
 
</P>
 
<br>
 
  
<p>
+
<p>
<a href="http://parts.igem.org/Part:BBa_K2354011">K2354011</a></td>
+
<a href="http://parts.igem.org/Part:BBa_K2354011">K2354011</a></td>
</P>
+
</P>
  
<p>
+
<p>
<img src="https://static.igem.org/mediawiki/2017/e/eb/T--NTHU_Taiwan--Parts--k11.png">
+
<img src="https://static.igem.org/mediawiki/2017/e/eb/T--NTHU_Taiwan--Parts--k11.png">
</p>
+
</p>
<br>
+
  
<p>
 
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of <I>E. coli</I> via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
 
</P>
 
<br>
 
  
<p>
+
<p>
<a href="http://parts.igem.org/Part:BBa_K2354012">K2354012</a></td>
+
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of <I>E. coli</I> via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
</P>
+
</P>
  
<p>
 
<img src="https://static.igem.org/mediawiki/2017/0/06/T--NTHU_Taiwan--Parts-k12.png">
 
</p>
 
<br>
 
  
<p>
+
<p>
Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
+
<a href="http://parts.igem.org/Part:BBa_K2354012">K2354012</a></td>
</P>
+
</P>
<br>
+
  
</center>
+
<p>
</body>
+
<img src="https://static.igem.org/mediawiki/2017/0/06/T--NTHU_Taiwan--Parts-k12.png">
 +
</p>
  
 
</div>
 
  
<script type="Text/JavaScript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.6/jquery.min.js"></script>
+
<p>
 +
Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
 +
</P>
  
<script>
 
  
// This is the jquery part of your template. 
 
// Try not modify any of this code too much since it makes your menu work.
 
  
$(document).ready(function() {
 
  
$("#HQ_page").attr('id','');
 
  
// call the functions that control the menu
 
menu_functionality();
 
hide_show_menu();
 
  
  
  
//this function controls the expand and collapse buttons of the menu and changes the +/- symbols
+
</div>
function menu_functionality() {
+
</div>
 
+
//when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
+
$(".menu_button").click(function(){
+
 
+
// add or remove the class "open" , this class holds the "-"
+
$(this).children().toggleClass("open");
+
// show or hide the submenu
+
$(this).next('.submenu_wrapper').fadeToggle(400);
+
});
+
 
+
// when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
+
$("#display_menu_control").click(function(){
+
$('#menu_content').fadeToggle(400);
+
});
+
 
+
// call the current page highlight function
+
highlight_current_page();
+
}
+
 
+
 
+
// call the highlight current page function to show it on the menu with a different background color
+
function highlight_current_page() {
+
 
+
// select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
+
$("#"+  wgPageName.substring(wgPageName.lastIndexOf("/")+1, wgPageName.length ) + "_page").addClass("current_page");
+
 
+
// now that the current_page class has been added to a menu item, make the submenu fade in
+
$(".current_page").parents(".submenu_wrapper").fadeIn(400);
+
// change the +/- symbol of the corresponding menu button
+
$(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
+
+
}
+
 
+
 
+
 
+
// allow button on the black menu bar to show/hide the side menu
+
function hide_show_menu() {
+
+
// in case you preview mode is selected, the menu is hidden for better visibility
+
if (window.location.href.indexOf("submit") >= 0) {
+
$(".igem_2017_menu_wrapper").hide();
+
}
+
 
+
// if the black menu bar has been loaded
+
  if (document.getElementById('bars_item')) {
+
 
+
// when the "bars_item" has been clicked
+
$("#bars_item").click(function() {
+
$("#sideMenu").hide();
+
 
+
// show/hide the menu wrapper
+
$(".igem_2017_menu_wrapper").fadeToggle("100");
+
});
+
  }
+
 
+
// because the black menu bars loads at a different time than the rest of the page, this function is set on a time out so it can run again in case it has not been loaded yet
+
else {
+
    setTimeout(hide_show_menu, 15);
+
}
+
}
+
 
+
 
+
});
+
 
+
 
+
</script>
+
  
 
</html>
 
</html>

Revision as of 12:47, 31 October 2017

Composite Parts



We constructed three composite parts and all of their function have been validated:Results

                             
biobricktype name description designer length
K2354010composite monobody-GBP HS Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface. Chan, Wei-Jen 850
K2354011composite INP-ER-alpha HS Expression of ER-alpha on the membrane of E. coli and ER-alpha can bind to EDCs Chan, Wei-Jen 2284
K2354012composite HRP-his tag Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1325

K2354010

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.

K2354011

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of E. coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.

K2354012

Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.