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<p>We want to construct a smart system. Only when the concentration of the lanthanide ions in the environment reaches the threshold, it reacts. </p> | <p>We want to construct a smart system. Only when the concentration of the lanthanide ions in the environment reaches the threshold, it reacts. </p> | ||
<p>In order to reach our goal, we need a smart sensing part. We found a two-component system called PmrA/PmrB. This system itself is aimed at iron ions. But if we can find the appropriate sequence which can react to lanthanide ions, we can replace the iron-binding with our own sequence and achieve our goal of sensing. | <p>In order to reach our goal, we need a smart sensing part. We found a two-component system called PmrA/PmrB. This system itself is aimed at iron ions. But if we can find the appropriate sequence which can react to lanthanide ions, we can replace the iron-binding with our own sequence and achieve our goal of sensing. | ||
− | <p>Fortunately, we did find it! Through the search, we know the most successful lanthanide | + | <p>Fortunately, we did find it! Through the search, we know the most successful lanthanide binder is lanthanide-binding tag.We will call it LBT in the following. After replacing, we fuse the pmrC promoter to the <I>gfp</I> gene which encodes green fluorescent protein to prove whether or not the PmrA-B-LBT are responsive to lanthanide.</p> |
− | <p>Ions sensing is just the first step, then we need to capture them. In this part, we also | + | <p>Ions sensing is just the first step, then we need to capture them. In this part, we also use LBT——our good lanthanide hunter.</p> |
<p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> | <p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> | ||
<p>Now our bacteria have collected a lot of lanthanide ions. What we need to do at last is just capturing our cells. So we used the Si-tag which we did in the previous project of our team to combine the bacteria with silicon to capture our bacteria.</p> | <p>Now our bacteria have collected a lot of lanthanide ions. What we need to do at last is just capturing our cells. So we used the Si-tag which we did in the previous project of our team to combine the bacteria with silicon to capture our bacteria.</p> |
Revision as of 17:03, 31 October 2017