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<p>We want to construct an intelligent system. Only when the concentration of the lanthanide ions reach the threshold, does it react. </p> | <p>We want to construct an intelligent system. Only when the concentration of the lanthanide ions reach the threshold, does it react. </p> | ||
<p>In order to reach our goal, we need a smart sensing circuit. Fortunately,we found a two-component system called pmrA/pmrB. This system itself is aimed at detecting iron ions. If we can find appropriate sequence which can react to lanthanide ions, we can replace the iron-binding sequence with them to achieve our goal. | <p>In order to reach our goal, we need a smart sensing circuit. Fortunately,we found a two-component system called pmrA/pmrB. This system itself is aimed at detecting iron ions. If we can find appropriate sequence which can react to lanthanide ions, we can replace the iron-binding sequence with them to achieve our goal. | ||
− | <p> Through consulting a large number of literature, we made it! We know the most successful lanthanide binder is lanthanide-binding tag.And we will call it LBT in the following. After exchanging sequences, we fuse the pmrC promoter to the <I>gfp</I> gene which encodes green fluorescent protein to prove whether or not the | + | <p> Through consulting a large number of literature, we made it! We know the most successful lanthanide binder is lanthanide-binding tag.And we will call it LBT in the following. After exchanging sequences, we fuse the pmrC promoter to the <I>gfp</I> gene which encodes green fluorescent protein to prove whether or not the pmrA-B-LBT are responsive to lanthanide.</p> |
<p>Ions sensing is just the first step, then we need to capture them. In this part, we also use LBT——our good lanthanide hunter.</p> | <p>Ions sensing is just the first step, then we need to capture them. In this part, we also use LBT——our good lanthanide hunter.</p> | ||
<p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> | <p>In order to capture more efficiently, we repeat them three times on cell with GS-Linker between each.</p> |
Revision as of 18:50, 31 October 2017