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− | <p>Firstly, we need to identify that OprF-3* LBT4 and OpRF-Sitag are expressed on the surface of the E. coli. We concentrated cell culture solution of induced test | + | <p>Firstly, we need to identify that OprF-3* LBT4 and OpRF-Sitag are expressed on the surface of the E. coli. We concentrated cell culture solution of induced test BL21 cells, As control, we also has the not induced BL21 cells' concentrated solution. Then we separate the protein of the whole cell by SDS-PAGE. </p> |
<p>Figure 1 shows an obvious ~33kDa protein bands in test lane, which cannot be found in the control lane. This result proves that the test BL21 can produce OprF-3* LBT 4. </p> | <p>Figure 1 shows an obvious ~33kDa protein bands in test lane, which cannot be found in the control lane. This result proves that the test BL21 can produce OprF-3* LBT 4. </p> | ||
<p>Figure 2 shows an obvious ~57kDa protein bands in test lane, which cannot be found in the not induced lane. This result proves that the test BL21 can produce OprF-Sitag. </p> | <p>Figure 2 shows an obvious ~57kDa protein bands in test lane, which cannot be found in the not induced lane. This result proves that the test BL21 can produce OprF-Sitag. </p> |
Revision as of 21:31, 31 October 2017