Experiments: Overview

• Transcriptome analysis of T. solium
  1. RNA Isolation
  2. RNA sequencing
• Bioinformatics
• Switch Synthesis
  1. Extension PCR
  2. Nested PCR
  3. Colony PCR
  4. Gel Electrophoresis
  5. DNA Clean-Up

• Toehold Switch Sensor Test – Testing Pipeline
  1. High troughput screening in liquid medium
  2. Reliable cell free expression on cellulose membranes
• Optimizing the Screening Pipeline
• Other Experiments
  1. 1. Preparation of nucleic acids (DNA preparation, Restriction digest/ DNA ligation, in vitro transcription, RNA purification)
  2. 2. Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)

Please find detailed protocols under the "Protocol" Section