Difference between revisions of "Team:MSU-Michigan/Measurement"

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/*character for gfp maturation*/
 
/*character for gfp maturation*/
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<div class="clear"></div>
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<div class="column side"></div>
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<div class="column middle">
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<h1>GFP Fluorescence Measurement Study</h1>
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</div>
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<head>
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<meta charset="UTF-8">
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<title>MSU iGEM character demo</title>
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<script src="https://code.jquery.com/jquery-3.2.1.min.js"> //allow your page to use jQuery
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</script>
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<script>
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$(function() {
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$('.myDIV').hover( //when you hover over the figure
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function() {
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$(this).append($("<img id='char' src='https://static.igem.org/mediawiki/2017/thumb/4/41/MSU-Michigan_fluorescenestdcurvedscrptn.png/800px-MSU-Michigan_fluorescenestdcurvedscrptn.png' />").hide().fadeIn()); //show the character
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}, function() {
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$('#char').remove(); //get rid of it after moving the cursor away from the figure
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});
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});
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</script>
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</head>
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<!--
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/*character for 0 hour graph*/
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-->
 
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<img class="two" src='img src="https://static.igem.org/mediawiki/2017/5/5e/MSU-Michigan_current_gfp%2Cgfpmtrb.png'/>
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         height:150px;
 
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         width:300px;
 
}
 
}
 
</style>
 
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<body>
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<div class="row">
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<div class="myDIV" style="position:left">
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<img class="two" src='https://static.igem.org/mediawiki/2017/a/ab/MSU-Michigan_GFP_maturation_gfp%2C_gfpmtrb.png' />
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</div> 
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<div class="myDIV0" style="position:left">
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<img class="two" src='https://static.igem.org/mediawiki/2017/2/2a/MSU-Michigan_gfphour0.png' />
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</div>
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</div>
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<div class="column side"></div>
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</div>
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</div>
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</body>
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</html>
 
</html>

Revision as of 22:57, 31 October 2017

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Measurement

Using Current for Protein Expression


MSU-iGEM 2017 developed a measurement tool that researchers throughout synthetic biology can utilize. Green fluorescent proteins (GFP) are used under aerobic conditions to signal protein expression. Unfortunately, for researchers working under anaerobic conditions, GFP does not mature and thus fluoresce under anaerobic conditions. Therefore, we showed that current induced via IPTG produces a reproducible signal for protein expression under anaerobic conditions. Current induction occurs 18 min after IPTG is added displaying a more rapid response than GFP expression. This measurement tool even proves to be more efficient than GFP expression under aerobic conditions due to the rapid current increased after induction. Also, current can be measured using simpler measuring tools than GFP expression. MSU-iGEM’s simplistic bioreactors utilizing Arduino boards are a model example of a system that can quantitatively measure protein expression via current increase in a more versatile way than using tools for measuring GFP fluorescence.

/*character for gfp maturation*/

GFP Fluorescence Measurement Study

MSU iGEM character demo