Difference between revisions of "Team:Stony Brook/Notebook/October"
| Line 112: | Line 112: | ||
<h4>9/03:</h4> | <h4>9/03:</h4> | ||
<p> • MiniPrep iGEM plasmids</p> | <p> • MiniPrep iGEM plasmids</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--Stony_Brook--13.1.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/a3/T--Stony_Brook--13.1.jpg" style="text-align: center;width:450px;height:250px;"/></a> |
<p> • RE Digest of the iGEM plasmids</p> | <p> • RE Digest of the iGEM plasmids</p> | ||
| Line 121: | Line 121: | ||
<h4>9/06:</h4> | <h4>9/06:</h4> | ||
<p> • Nanodrop of gel extract</p> | <p> • Nanodrop of gel extract</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/69/T--Stony_Brook--13.2.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/69/T--Stony_Brook--13.2.jpg" style="text-align: center;width:450px;height:250px;"/></a> |
<p> • Miniprep small liquid cultures of constructs</p> | <p> • Miniprep small liquid cultures of constructs</p> | ||
<a href="#"><img src="https://static.igem.org/mediawiki/2017/d/dc/T--Stony_Brook--13.3.jpg" style="text-align: center;width:250px;height:250px;"/></a> | <a href="#"><img src="https://static.igem.org/mediawiki/2017/d/dc/T--Stony_Brook--13.3.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
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<h4>9/11:</h4> | <h4>9/11:</h4> | ||
<p> • BioBrick MiniPrep Nanodrop</p> | <p> • BioBrick MiniPrep Nanodrop</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/68/T--Stony_Brook--13.4.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/68/T--Stony_Brook--13.4.jpg" style="text-align: center;width:450px;height:250px;"/></a> |
<h4>10/13:</h4> | <h4>10/13:</h4> | ||
Revision as of 01:52, 1 November 2017
Weeks 13-17
8/21:
• Prep for testing
8/22: MRSA Testing day 1
• Hydrate MRSA and Staphylococcus Aureus
• Create liquid cultures in preparation for testing
• Gel Extraction of two samples each of lnqZE, lnqZA, lnqZQ, lnq Z
8/23: MRSA testing day 2
• Performed zone of inhibition test
8/24: MRSA testing day 3
• Analyzed results of the zone of inhibition test
• Performed test to see if any protein would kill a liquid culture of the cells
• Gel Extraction of two samples each of Z+E, Z+A, ZQ, Lnq Z
8/25:
• Analyzed the results
• Killed all MRSA and Staphylococcus aureus samples
• Disinfected lab
9/01:
• Transform DH5ɑ cells with iGEM plasmids and plate.
9/02:
• Make small liquid cultures of both plasmid 1 and plasmid 2
9/03:
• MiniPrep iGEM plasmids
• RE Digest of the iGEM plasmids
9/05:
• Ran digests on gel and gel extracted
• Make small liquid cultures of GST Epi, GST A53, GST Lnq Z, MBP Z+A, and MBP Z+Q
9/06:
• Nanodrop of gel extract
• Miniprep small liquid cultures of constructs
9/11:
• BioBrick MiniPrep Nanodrop
10/13:
• Gel
10/18:
• Restriction Enzyme Digest of Aureocin A53 and Epi
• Prep BioBricks
10/22:
• Dot Blot
10/23:
• Dot Blot
