Measurement

Using Current for Protein Expression

MSU-iGEM 2017 developed a measurement tool that researchers throughout synthetic biology can utilize. Green fluorescent proteins (GFP) are used under aerobic conditions to signal protein expression (1,4,6). Unfortunately, for researchers working under anaerobic conditions, GFP does not mature and thus fluoresce under anaerobic conditions (2). Therefore, we showed that current induced via IPTG produces a reproducible signal for protein expression under anaerobic conditions. Current induction occurs 18 min after IPTG is added displaying a more rapid response than GFP expression. This measurement tool even proves to be more efficient than GFP expression under aerobic conditions due to the rapid current increased after induction. Also, current can be measured using simpler measuring tools than other anaerobic protein expression tools (3, 5, 7). MSU-iGEM’s simplistic bioreactors utilizing Arduino boards are a model example of a system that can quantitatively measure protein expression via current increase in a more versatile way than using tools for measuring GFP fluorescence.

GFP Fluorescence Measurement Study

Discussion

References

(1) Chiu Wan-Ling; Niwa, Y.; Zeng, W.; Hirano, T.; Kobayashi, H.; Sheen, J. Curr. Biol. 1996, 6 (3), 325–330.
(2) Drepper, T.; Eggert, T.; Circolone, F.; Heck, A.; Krauß, U.; Guterl, J.-K.; Wendorff, M.; Losi, A.; Gärtner, W.; Jaeger, K.-E. Nat. Biotechnol. 2007, 25 (4), 443–445.
(3) Fuchs, S.; Pané-Farré, J.; Kohler, C.; Hecker, M.; Engelmann, S. J. Bacteriol. 2007, 189 (11), 4275–4289.
(4) Heim, R.; Prasher, D. C.; Tsien, R. Y. Proc. Natl. Acad. Sci. 1994, 91 (26), 12501–12504.
(5) Mus, F.; Dubini, A.; Seibert, M.; Posewitz, M. C.; Grossman, A. R. J. Biol. Chem. 2007, 282 (35), 25475–25486.
(6) Scholz, O.; Thiel, A.; Hillen, W.; Niederweis, M. Eur. J. Biochem. 2000, 267 (6), 1565–1570.
(7) Sørensen, H. P.; Mortensen, K. K. Journal of Biotechnology. 2005, pp 113–128.

Sponsors
										Contact us: