Difference between revisions of "Team:SCU China"
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<p class="scu_title">Rhythmic Production of Melatonin in <i>E.coli</i></p> | <p class="scu_title">Rhythmic Production of Melatonin in <i>E.coli</i></p> | ||
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<div class="scu_down" id="scu_down"> | <div class="scu_down" id="scu_down"> | ||
<p class="scu_down">learn more about us</p> | <p class="scu_down">learn more about us</p> | ||
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<div class="overview-sub-title">SCU_China</div> | <div class="overview-sub-title">SCU_China</div> | ||
| + | <p class="scu_main_page_content_p2"> | ||
| + | Nowadays, insomnia and circadian rhythm disorders are increasingly plaguing | ||
| + | individuals' daily life. The circadian rhythm in human is predominantly under the | ||
| + | regulation of melatonin, a hormone produced by pineal gland periodically in | ||
| + | accordance with day-and-night photoperiod. This year we want to use engineered | ||
| + | <i>E.coli</i> to mimic this process in human as a promising dosing machine. We propose to | ||
| + | <strong>construct the melatonin biosynthesis pathway in <i>E.coli</i> to produce melatonin</strong>, and | ||
| + | simultaneously <strong>couple this process with optimized repressilator</strong>, a synthetic genetic | ||
| + | oscillator with higher precision and stability, to magnify the function of | ||
| + | repressilator and <strong>render melatonin production resemble mammalian periodicity in | ||
| + | <i>E.coli</i></strong>. | ||
| + | </p> | ||
| + | </div> | ||
| + | <div class="scu_hp_image_single_main"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/7/7d/T-SCU_China-2017_Team_fig1.jpg"> | ||
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1. Our human practices were integrated to the whole process, which made us | 1. Our human practices were integrated to the whole process, which made us | ||
complete | complete | ||
| − | the project better. | + | the project better.<br> |
2. We improved two previous part BBa_I13521 and BBa_E2050 and our BioBrick work | 2. We improved two previous part BBa_I13521 and BBa_E2050 and our BioBrick work | ||
well | well | ||
| − | in lab condition. | + | in lab condition.<br> |
3. Our experimental design based on modelling results, such as the optimal | 3. Our experimental design based on modelling results, such as the optimal | ||
| − | proportioning of synthetase. | + | proportioning of synthetase.<br> |
4. Engineered E.coli is cultured with artificial gastrointestinal juices and gut | 4. Engineered E.coli is cultured with artificial gastrointestinal juices and gut | ||
flora to validate the substantial integration and survival condition based on | flora to validate the substantial integration and survival condition based on | ||
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<p class="scu_main_page_content_p" id="middle"> | <p class="scu_main_page_content_p" id="middle"> | ||
1. There are 4 new BioBrick parts in our project as follow, BBa_K2276001, | 1. There are 4 new BioBrick parts in our project as follow, BBa_K2276001, | ||
| − | BBa_K2276003, BBa_K2276004 and BBa_K2206005. | + | BBa_K2276003, BBa_K2276004 and BBa_K2206005.<br> |
2. We collaborated with SCU-WestChina and SiCAU-China in experiments and human | 2. We collaborated with SCU-WestChina and SiCAU-China in experiments and human | ||
practice, received key assists from Peking University and offered wiki design | practice, received key assists from Peking University and offered wiki design | ||
| − | suggestions to team Purdue. | + | suggestions to team Purdue.<br> |
3. We consulted experts of China Food and Drug Administration and West China | 3. We consulted experts of China Food and Drug Administration and West China | ||
Sleeping Centre for both experimental designs and safety problems. Also, we | Sleeping Centre for both experimental designs and safety problems. Also, we | ||
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<div class="scu_main_page_content_wrapper" id="right_wrapper"> | <div class="scu_main_page_content_wrapper" id="right_wrapper"> | ||
| − | <p | + | <p class="scu_main_page_content_p" id="right"> |
| − | 1. We constructed a team successfully, sharing not only academic knowledge but also | + | 1. We constructed a team successfully, sharing not only academic knowledge but |
| − | life experiences with each other. | + | also |
| + | life experiences with each other.<br> | ||
2.We received many kindly help from surroundings, including our college, | 2.We received many kindly help from surroundings, including our college, | ||
| − | instructor, experts in various fields, friendly fraternal teams and so on.We have clearly acknowledged their works. | + | instructor, experts in various fields, friendly fraternal teams and so on.We |
| − | 3. We managed to meet all the Deliverables standards. | + | have clearly acknowledged their works.<br> |
| + | 3. We managed to meet all the Deliverables standards.<br> | ||
4. We participated in the Interlab Measurement Study. | 4. We participated in the Interlab Measurement Study. | ||
<br><br><br> | <br><br><br> | ||
Revision as of 02:38, 1 November 2017
Rhythmic Production of Melatonin in E.coli
learn more about us
Nowadays, insomnia and circadian rhythm disorders are increasingly plaguing individuals' daily life. The circadian rhythm in human is predominantly under the regulation of melatonin, a hormone produced by pineal gland periodically in accordance with day-and-night photoperiod. This year we want to use engineered E.coli to mimic this process in human as a promising dosing machine. We propose to construct the melatonin biosynthesis pathway in E.coli to produce melatonin, and simultaneously couple this process with optimized repressilator, a synthetic genetic oscillator with higher precision and stability, to magnify the function of repressilator and render melatonin production resemble mammalian periodicity in E.coli.
1. Our human practices were integrated to the whole process, which made us
complete
the project better.
2. We improved two previous part BBa_I13521 and BBa_E2050 and our BioBrick work
well
in lab condition.
3. Our experimental design based on modelling results, such as the optimal
proportioning of synthetase.
4. Engineered E.coli is cultured with artificial gastrointestinal juices and gut
flora to validate the substantial integration and survival condition based on
the
consideration for better safety and higher efficiency.
1. There are 4 new BioBrick parts in our project as follow, BBa_K2276001,
BBa_K2276003, BBa_K2276004 and BBa_K2206005.
2. We collaborated with SCU-WestChina and SiCAU-China in experiments and human
practice, received key assists from Peking University and offered wiki design
suggestions to team Purdue.
3. We consulted experts of China Food and Drug Administration and West China
Sleeping Centre for both experimental designs and safety problems. Also, we
collected and analyzed convincing datas from our survey.
1. We constructed a team successfully, sharing not only academic knowledge but
also
life experiences with each other.
2.We received many kindly help from surroundings, including our college,
instructor, experts in various fields, friendly fraternal teams and so on.We
have clearly acknowledged their works.
3. We managed to meet all the Deliverables standards.
4. We participated in the Interlab Measurement Study.
