Difference between revisions of "Team:OUC-China/Improve"
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<h1><strong>Improve</strong></h1> | <h1><strong>Improve</strong></h1> | ||
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| − | < | + | <h2 class="ouc-heading"><strong>Inspiration</strong></h2> |
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<img src="https://static.igem.org/mediawiki/2017/thumb/a/aa/T--OUC-China--improve.illustration.png/800px-T--OUC-China--improve.illustration.png" width="700px"/> | <img src="https://static.igem.org/mediawiki/2017/thumb/a/aa/T--OUC-China--improve.illustration.png/800px-T--OUC-China--improve.illustration.png" width="700px"/> | ||
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<p style="text-align: center"> | <p style="text-align: center"> | ||
<img src="https://static.igem.org/mediawiki/2017/thumb/7/77/T--OUC-China--improve.FI_Abs600.png/800px-T--OUC-China--improve.FI_Abs600.png" width="700px"/> | <img src="https://static.igem.org/mediawiki/2017/thumb/7/77/T--OUC-China--improve.FI_Abs600.png/800px-T--OUC-China--improve.FI_Abs600.png" width="700px"/> | ||
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as a generic enhancement module. | as a generic enhancement module. | ||
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| − | < | + | <h2 class="ouc-heading"><strong>Reference</strong></h2> |
[1]Zhou, Shenghu, et al. "Obtaining a Panel of Cascade Promoter-5′-UTR Complexesin Escherichia coli." Acs Synthetic Biology 6.6(2017)<br/> | [1]Zhou, Shenghu, et al. "Obtaining a Panel of Cascade Promoter-5′-UTR Complexesin Escherichia coli." Acs Synthetic Biology 6.6(2017)<br/> | ||
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Revision as of 12:29, 1 November 2017
Improve
Inspiration
5'UTR will be transcribed and can be used as a regulatory element to adjust
the translation process. Thus, there is reason to believe that a delicate
structure of the 5'UTR has the potential as a universal enhancement
module. We see that Zhou et al. Screened some 5'UTR sequences from E.
coli, which helped to improve protein content. Thus, we are prepared to
consider this part as an enhancement module, the idea also received the
author's support.[1]
Design
We selected J23108 from constitutive promoter family members and added the 5'UTR sequence thereafter. Fluorescent protein is used to characterize whether the 5'UTR has an enhanced effect on the promoter.
Proof of concept
We constructed two red fluorescent protein devices and constructed the loop by randomly selecting the promoter and the terminator. The difference is that UTRrpsT is added to a loop and the fluorescence intensity is measured using a microplate reader and plotted. We found that the fluorescence intensity of 6h increased by 1.5 fold. This indicates that UTRrpsT has the capability as a generic enhancement module.
Reference
[1]Zhou, Shenghu, et al. "Obtaining a Panel of Cascade Promoter-5′-UTR Complexesin Escherichia coli." Acs Synthetic Biology 6.6(2017)
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