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US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which | US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which | ||
is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, | is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, | ||
− | high energy consumption and a lack of sustainablility.<br> This year, we aim to use a greener and more environmentally- | + | high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally- |
friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional | friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional | ||
chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene. | chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene. | ||
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<h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type | ||
is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic | is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic | ||
− | effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) | + | effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) |
platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) | platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) | ||
and HTS(High throughput screening). | and HTS(High throughput screening). | ||
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optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol | optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol | ||
metabolism of the two organisms, so that they could produce the target product acrylic acid using the | metabolism of the two organisms, so that they could produce the target product acrylic acid using the | ||
− | intermediates G3P and DHAP. Besides the construction of the pathways, we also reconstructed | + | intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed |
and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
and reduced the loss of by-pass carbon flux.</h4> | and reduced the loss of by-pass carbon flux.</h4> | ||
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<h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic | <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic | ||
acid. In the subsequent fermentation, we also determined the best conditions | acid. In the subsequent fermentation, we also determined the best conditions | ||
− | of the engineered microorganism strains.<br> Therefore, we selected to control the carbon source, buffer, temperature, pH | + | of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH |
and other conditions to optimize the cell production process.</h4> | and other conditions to optimize the cell production process.</h4> | ||
</div> | </div> |
Latest revision as of 12:52, 1 November 2017