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Revision as of 13:03, 1 November 2017
The funniest things
Lost in translation: The floppy disk and the USB drive
For the last part of our project we had to introduce Gallium-Siderophore complexes to our bacteria and the respective human cell lines. Since complexation is not a given, and it is necessary to proof that the complex has formed. One of our main goals was to provide evidence that already complexed siderophores are more effective than just applying gallium and the siderophores together.
To generate the necessary evidence, we wanted to measure kinetics of the complex formation to show that a complex is forming. Luckily our products are photoactive so that we could monitor it with a standard photometer over a couple of hours. To find out which percentile of our siderophores formed a complex we applied NMR-measurements as well.
However, since the Computer that was attached to the photometer was old, running on Win 98 to be precise, we had some issues extracting our data from it.
And this is where the story begins:
Once upon a time, 10 days ahead of the giant jamboree, a chemist wandered into an old laboratory. To measure UV-absorption of a former unknown chemical compound. Unwavering he approached the good old photometer and its translator Dell Optiplex Win 98, a quartz cuvette filled with pyochelin his only companion. He applied a small amount of gallium-nitrate solution to the pyochelin and started a kinetics measurement to find out what intrigued him. The measurements went just fine the chemist was relieved and happy. Then he said:” Well the data is looking good, now I just have to analyse it in detail and therefore extract the date from Optiplex”.
So, he grabbed his USB-drive and connected it to Optiplex, Optiplex replied wtf is that. The chemist said well this is a modern data storage system give me my data. Optiplex replied wtf is that, give me a driver. Chemist replied well provide me with internet then. Optiplex replied no. The chemist who was slightly annoyed already said well then what can I do. After an inquiry with the IT-service of the university he was brought to an old dusty cardboard box, there you will find what you are seeking my friend the IT-expert said. The chemist opened the old and dusty box and was quite surprised by what he found, a single, gray floppy disk.
Armed with his USB-drive and the floppy disk he went back to face Optiplex again. Optiplex seemed quite content with what he was offered. The data could be transferred without any further problems. However, another question arose how can we bring the floppy disk together with the UBS drive. Luckily the answer to the question hid just about 10 m away on the other side of the floor.
In room 110 another old machine was found, his exterior so scratched and battered that his name was unreadable. The chemist approached it cautiously, unsettled by his earlier experience, but voila both the USB-drive and the floppy disk were readable and finally together and the chemist finally relieved could start his detailed analysis.
For the last part of our project we had to introduce Gallium-Siderophore complexes to our bacteria and the respective human cell lines. Since complexation is not a given, and it is necessary to proof that the complex has formed. One of our main goals was to provide evidence that already complexed siderophores are more effective than just applying gallium and the siderophores together.
To generate the necessary evidence, we wanted to measure kinetics of the complex formation to show that a complex is forming. Luckily our products are photoactive so that we could monitor it with a standard photometer over a couple of hours. To find out which percentile of our siderophores formed a complex we applied NMR-measurements as well. However, since the Computer that was attached to the photometer was old, running on Win 98 to be precise, we had some issues extracting our data from it.
And this is where the story begins:
Once upon a time, 10 days ahead of the giant jamboree, a chemist wandered into an old laboratory. To measure UV-absorption of a former unknown chemical compound. Unwavering he approached the good old photometer and its translator Dell Optiplex Win 98, a quartz cuvette filled with pyochelin his only companion. He applied a small amount of gallium-nitrate solution to the pyochelin and started a kinetics measurement to find out what intrigued him. The measurements went just fine the chemist was relieved and happy. Then he said:” Well the data is looking good, now I just have to analyse it in detail and therefore extract the date from Optiplex”.
So, he grabbed his USB-drive and connected it to Optiplex, Optiplex replied wtf is that. The chemist said well this is a modern data storage system give me my data. Optiplex replied wtf is that, give me a driver. Chemist replied well provide me with internet then. Optiplex replied no. The chemist who was slightly annoyed already said well then what can I do. After an inquiry with the IT-service of the university he was brought to an old dusty cardboard box, there you will find what you are seeking my friend the IT-expert said. The chemist opened the old and dusty box and was quite surprised by what he found, a single, gray floppy disk.
Armed with his USB-drive and the floppy disk he went back to face Optiplex again. Optiplex seemed quite content with what he was offered. The data could be transferred without any further problems. However, another question arose how can we bring the floppy disk together with the UBS drive. Luckily the answer to the question hid just about 10 m away on the other side of the floor.In room 110 another old machine was found, his exterior so scratched and battered that his name was unreadable. The chemist approached it cautiously, unsettled by his earlier experience, but voila both the USB-drive and the floppy disk were readable and finally together and the chemist finally relieved could start his detailed analysis.
