Difference between revisions of "Team:UCLouvain/Composite Part"
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<div class="site-hero_2"> | <div class="site-hero_2"> | ||
<div class="page-title"> | <div class="page-title"> | ||
| − | <div class="big-title montserrat-text uppercase"> | + | <div class="big-title montserrat-text uppercase">in the lab</div> |
| − | <div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;"> | + | <div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite parts</Marine></div> |
</div> | </div> | ||
</div> | </div> | ||
<section> | <section> | ||
| − | + | <div class="container"> | |
| + | <div class="row" style="margin-bottom: 40px;"> | ||
| + | |||
| + | <div class="col-md-12"> | ||
| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <span>pCMMCD 105</span> | ||
| + | </div> | ||
| + | <BR><BR> | ||
| + | |||
| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <p class="montserrat-text uppercase">Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p> | ||
| + | </div> | ||
| + | <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a much used reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and <i>para</i>-nitrophenyl glucuronide. | ||
| + | </p> | ||
| + | <p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL). | ||
| + | </p> | ||
| + | <div class="container"> | ||
| + | <div class="row" style="margin-bottom: 40px;"> | ||
| + | |||
| + | <div class="col-md-12"> | ||
| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <span>piFinal (BBa_K577882)</span> | ||
| + | </div> | ||
| + | <BR><BR> | ||
| + | |||
| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <p class="montserrat-text uppercase">iGEM plasmid consisting of araC, a pBAD promoter, a RBS, a RFP and two terminators.</p> | ||
| + | </div> | ||
| + | |||
| + | |||
</section> | </section> | ||
</html> | </html> | ||
{{UCLouvainFooter}} | {{UCLouvainFooter}} | ||
Revision as of 13:38, 1 November 2017
in the lab
pCMMCD 105
Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.
GusA encodes the beta-glucuronidase gene from E. coli. This is a much used reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.
Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).
piFinal (BBa_K577882)
iGEM plasmid consisting of araC, a pBAD promoter, a RBS, a RFP and two terminators.
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