Difference between revisions of "Team:UCLouvain/Composite Part"
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<p class="montserrat-text uppercase">Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p> | <p class="montserrat-text uppercase">Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p> | ||
</div> | </div> | ||
| − | <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a | + | <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and <i>para</i>-nitrophenyl glucuronide. |
</p> | </p> | ||
<p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL). | <p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL). | ||
Revision as of 13:40, 1 November 2017
in the lab
pCMMCD 105
Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.
GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.
Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).
piFinal (BBa_K577882)
iGEM plasmid consisting of araC, a pBAD promoter, a RBS, a RFP and two terminators.
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