Difference between revisions of "Team:UCLouvain/Composite Part"
| Line 6: | Line 6: | ||
<div class="page-title"> | <div class="page-title"> | ||
<div class="big-title montserrat-text uppercase">in the lab</div> | <div class="big-title montserrat-text uppercase">in the lab</div> | ||
| − | <div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite | + | <div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite Parts</Marine></div> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:41, 1 November 2017
in the lab
pCMMCD 105
Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.
GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.
Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).
piFinal (BBa_K577882)
iGEM plasmid consisting of araC, a pBAD promoter, a RBS, a RFP and two terminators.
you think we're cool? so are our sponsors!
