Difference between revisions of "Team:MSU-Michigan/Demonstrate"
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<a href="https://2017.igem.org/Team:MSU-Michigan/Current" class="w3-button w3-green w3-ripple w3-round w3-hover-white">Current Induction</a> | <a href="https://2017.igem.org/Team:MSU-Michigan/Current" class="w3-button w3-green w3-ripple w3-round w3-hover-white">Current Induction</a> | ||
</div> | </div> | ||
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| + | <div class="w3-cell-row"> | ||
| + | <div class="w3-col w3-container" style="width:17.5%"></div> | ||
| + | <div class="w3-col w3-container w3-pale-green" style="width:60%"> | ||
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| + | <div class="w3-cell-row w3-pale-green" style="width:100%"> | ||
| + | <div class="w3-container w3-cell"> | ||
| + | <div class="w3-card-4 w3-pale-blue" style="width:100%"> | ||
| + | <div class="w3-container w3-center"> | ||
| + | <h2>M5 Media Fluorescence</h2> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/c/c3/MSU-Michigan_IPTGInduction_GFP_M5.png" style="width:100%"> | ||
| + | <p> Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <div class="w3-container w3-cell"> | ||
| + | <div class="w3-card-4 w3-pale-blue" style="width:100%"> | ||
| + | <div class="w3-container w3-center"> | ||
| + | <h2>mtrB Induction Fluorescence</h2> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/8/8c/MSU-Michigan_IPTGInduction_GFP_mtrB.png" style="width:100%"> | ||
| + | <p> Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <div class="w3-cell-row w3-pale-green" style="width:100%"> | ||
| + | <div class="w3-container w3-cell"> | ||
| + | <div class="w3-card-4 w3-pale-blue" style="width:100%"> | ||
| + | <div class="w3-container w3-center"> | ||
| + | <h2>mtrB_GFP Induction Fluorescence</h2> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/8/82/MSU-Michigan_IPTGInduction_GFP_mtrB_GFP.png" style="width:100%"> | ||
| + | <p>Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. This is the highest because resources are not being directed toward the mtrB pathway, which is still removed</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | |||
| + | <div class="w3-container w3-cell"> | ||
| + | <div class="w3-card-4 w3-pale-blue" style="width:100%"> | ||
| + | <div class="w3-container w3-center"> | ||
| + | <h2>mrtB_GFP_mtrB Induction Fluorescence</h2> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/5/51/MSU-Michigan_IPTGInduction_GFP_mtrB_GFP_mtrB.png" style="width:100%"> | ||
| + | <p>Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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Revision as of 14:05, 1 November 2017
Demonstrate
MSU iGEM has succeeded in inducing current from induction of a selected chemical.
We measured the results using both IPTG and current.
Below is a video showing the working bioreactors outputting current.
M5 Media Fluorescence
Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.
mtrB Induction Fluorescence
Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control
mtrB_GFP Induction Fluorescence
Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. This is the highest because resources are not being directed toward the mtrB pathway, which is still removed
mrtB_GFP_mtrB Induction Fluorescence
Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.
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