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<h2>IPTG Induction</h2>
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<a href="https://2017.igem.org/Team:MSU-Michigan/Results" class="w3-button w3-xxlarge w3-padding-large w3-green w3-ripple w3-round w3-hover-white">IPTG Induction Results</a>
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<h2>M5 Media Fluorescence</h2>
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<p> Control (M5 Media only) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. </p>
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<h2>mtrB Induction Fluorescence</h2>
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<p> Induction of mtrB gene (no GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate. - Acts as a second control</p>
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<h2>mtrB_GFP Induction Fluorescence</h2>
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<p>Induction of mtrB_GFP strain (GFP, no mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.  This is the highest because resources are not being directed toward the mtrB pathway, which is still removed</p>
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<h2>mrtB_GFP_mtrB Induction Fluorescence</h2>
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  <p>Induction of mtrB_GFP_mtrB strain (GFP, mtrB insert) for proof of IPTG induction at different concentrations, measured by GFP fluorescence in a 96-well plate.</p>
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Revision as of 14:21, 1 November 2017

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Applied Design Hardware Measurement Model

Demonstrate

MSU iGEM has succeeded in inducing current from induction of a selected chemical.


We measured the results using both IPTG and current.


Below is a video showing the working bioreactors outputting current.

IPTG Induction Results

Current from Induction

deltamtrB_GFPmtrB Induced V Uninduced

Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.

deltamtrB_GFPmtrB Induced V Uninduced

The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.

Multiple Strains

Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.

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