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<li><a href="#section2">Reaction System<br> of Colony PCR</a></li> | <li><a href="#section2">Reaction System<br> of Colony PCR</a></li> | ||
<li><a href="#section3">SDS-PAGE</a></li> | <li><a href="#section3">SDS-PAGE</a></li> | ||
− | <li><a href="# | + | <li><a href="#section4">Titration</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
− | + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Titration</h3> | |
<p>We transferred the plasmids carrying the sequences encoding 3×LBT into BL21, and set a control group in which 3×LBT is not involved in the plasmids transferred. Both can be expressed with the induction of IPTG. Having cultivated the bacteria into similar densities, we add IPTG and then incubate them for 5 hours. Afterwards, we boiled the bacteria to release the proteins on their cell membrane. Then we detected the proteins we extracted by SDS-PAGE. As a result, bacteria in the test group expressed a large quantity of LBT while those in the control group did not. | <p>We transferred the plasmids carrying the sequences encoding 3×LBT into BL21, and set a control group in which 3×LBT is not involved in the plasmids transferred. Both can be expressed with the induction of IPTG. Having cultivated the bacteria into similar densities, we add IPTG and then incubate them for 5 hours. Afterwards, we boiled the bacteria to release the proteins on their cell membrane. Then we detected the proteins we extracted by SDS-PAGE. As a result, bacteria in the test group expressed a large quantity of LBT while those in the control group did not. | ||
</p> | </p> |
Revision as of 18:27, 1 November 2017