General Introduction

As described on the Design section, we developed a laboratory methodology to accomplish our proof of concept. Fortunately, thanks to different factors, this methodology helped us accomplish all of the bronze, silver, and gold requirements. For example, there were some parts that were already registered at iGEM but lacked characterization. Some others were reported as putative genes on NCBI, which gave us enough reasons to create new parts meeting the silver criteria. Finally, thanks to our Integrated Human Practices and Entrepreneurship, we were able to establish a demonstration protocol that meets the necessities of a client or posible investor like "La Norteñita" (leading company in apple production and commercialization).

The characterizations that were made were both at a molecular and cellular level. For example, we did an expression kinetic (bronze and silver), an adherence capacity test (improve), and an apple tree leaves inoculation protocol (demonstrate). You can read more of the results by clicking on the buttons below.