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which will continue to participate in the biosynthesis of clavulanic acid as the first intermediate.<br> | which will continue to participate in the biosynthesis of clavulanic acid as the first intermediate.<br> | ||
<center><img src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png" class="img-responsive"></center> | <center><img src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png" class="img-responsive"></center> | ||
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<br>According to the earlier literature, CEAS (N2-(2-carboxyethyl) arginine synthase) was a synergistic | <br>According to the earlier literature, CEAS (N2-(2-carboxyethyl) arginine synthase) was a synergistic | ||
effect of Ceas1 and Ceas, namely, N2- (2-carboxyethyl) arginine came from the condensation of G3P | effect of Ceas1 and Ceas, namely, N2- (2-carboxyethyl) arginine came from the condensation of G3P | ||
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of N2-(2-carboxyethyl)arginine synthase, the first enzyme in the clavulanic acid biosynthesis pathway | of N2-(2-carboxyethyl)arginine synthase, the first enzyme in the clavulanic acid biosynthesis pathway | ||
[J]. Journal of Biological Chemistry, 2004, 279(7): 5685-92.</h5> | [J]. Journal of Biological Chemistry, 2004, 279(7): 5685-92.</h5> | ||
− | + | <h2 style="text-align:center"> How we use these basic parts?</h2> | |
+ | <img src="https://static.igem.org/mediawiki/2017/c/ce/5%2C6%2C7%2C8%EF%BC%8C10.png" class="img-responsive"> | ||
+ | <center>Fig.5:DAK;6:NOX;7,ceaS2;8:gld;10:CAT</center> | ||
+ | <h4>We designed a GDC (GlyDH-DAK-Ceas2) pathway that could produce acrylic acid using glycerol. In this pathway, GlyDH (Glycerol dehydrogenase,BBa_K2347001) can efficiently convert glycerol to DHA (1,3-Dihydroxyacetone), and then DAK(Dihydroxyacetone kinase,BBa_K2347002) enzyme can phosphorylate DHA to DHAP, and then the ceaS2 Enzyme (BBa_K2347000)can make it into acrylic acid. | ||
+ | <br>In addition, since GlyDH is NAD + dependent, in order to increase the supply of reducing force, we also added the NOX (NADH dehydrogenase, BBa_K2347003) and CAT (Catalase, BBa_K2347004) to this pathway, providing the reduction force for GlyDH through two layers of substrate. Finally, we created the new combination of GNCDC (GlyDH-NOX-CAT-DAK-ceaS2) pathway using five enzymes from BBa_K2347000 to BBa_K2347004 to produce acrylic acid.</h4> | ||
</div> | </div> | ||
Revision as of 20:36, 1 November 2017
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