Difference between revisions of "Team:Berlin diagnostX/Experiments"

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         <h3 class="text-center igem_blue mt-4">Transcriptome Analysis of <em>T. solium</em></h3>
 
         <h3 class="text-center igem_blue mt-4">Transcriptome Analysis of <em>T. solium</em></h3>
 
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        <h5 class="igem_blue text-center pb-1">RNA Isolation</h5>
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        <p class="text-justify">Toehold switch sensors are based on synthetic biology. Essentially, they are RNA molecules which code for a reporter protein. They consist of a specific toehold sequence, a ribosome-binding site (which is important for the production of proteins) and a sequence for the reporter protein. The reporter protein can only be produced if the sensor has bonded with its specific target RNA sequence [4]. When producing the sensors, we can select both the toehold sequence and the reporter protein with complete flexibility to match our needs. In this way we can fashion our Wormspotter so that it only sends a desired signal when it binds to RNA molecules specific to T. solium. We are planning to use T. solium-specific RNA sequences for the toehold sequence and beta-Galactosidase as a reporter protein.</p>
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        <h5 class="igem_blue text-center pb-1">RNA Sequencing</h5>
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        <p class="text-justify">Toehold switch sensors are based on synthetic biology. Essentially, they are RNA molecules which code for a reporter protein. They consist of a specific toehold sequence, a ribosome-binding site (which is important for the production of proteins) and a sequence for the reporter protein. The reporter protein can only be produced if the sensor has bonded with its specific target RNA sequence [4]. When producing the sensors, we can select both the toehold sequence and the reporter protein with complete flexibility to match our needs. In this way we can fashion our Wormspotter so that it only sends a desired signal when it binds to RNA molecules specific to T. solium. We are planning to use T. solium-specific RNA sequences for the toehold sequence and beta-Galactosidase as a reporter protein.</p>
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Revision as of 20:55, 1 November 2017

Experiments