Difference between revisions of "Team:Gaston Day School/Notebook"
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Revision as of 20:57, 1 November 2017
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
MgCl:
| ??? | ??? | ??? |
|---|---|---|
| 14.3 | H2O | 114.4 |
| 2.5 | Buffer | 20 |
| 1 | MgCl2 | 8 |
| 1 | dNTP | 8 |
tube:
| 2ul | H2O |
| -5ul | F |
| -5ul | R |
Label:
| 0 | Control |
| 1 | AceF |
| 2 | EutG BB |
| 3 | YiaY BB |
| 4 | Adhp BB |
| 5 | Lpol |
| 6 | AceE |
Master Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| ??? | 1 | 8 |
| dH2O | 13.3 | 106.4 |
| ??? | 0.2 | 1.6 |
| / | dH2O | For | Rev | / |
|---|---|---|---|---|
| 1 | 4 | 0.5 | 0.5 | AceF |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdnP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 7 | 5 | - | - | - |
Master Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 12.3 | 98.4 |
| taq | 0.2 | 1.6 |
| MgCl2 | 1 | 8 |
| dH2O | For | Rev | ||
|---|---|---|---|---|
| 0 | 5 | - | - | |
| 1 | 4 | 0.5 | 0.5 | ACE F |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdhP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 1 | - | DNA(0) | dH2O(5) |
|---|---|---|---|
| 2 | J23102 | 3 | 2 |
| 3 | GlmY | 3 | 2 |
| 4 | A(1) | 3 | 2 |
| 5/td> | A(2) | 3 | 2 |
| 6 | B(2) | 3 | 2 |
| 7 | C(2) | 3 | 2 |
Max
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J23102B pp | 3 | 2 |
| 3 | J23102 P NNP | 3 | 2 |
| 4 | GlmY-3A | 3 | 2 |
| 5 | 102B+GlmY 1B | 3 | 2 |
| 6 | 102B+GlmY 1C | 3 | 2 |
| 7 | 102B_GlmY 1D | 3 | 2 |
Master Mix
| H2O | 13.3 | 106.4 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| GDNA | 3 | 24 |
| taq | 0.2 | 1.6 |
Gel:
| dH2O | For | Rev | ||
|---|---|---|---|---|
| 1 | 5 | - | - | |
| 2 | 4 | 0.5 | 0.5 | AceF |
| 3 | 4 | 0.5 | 0.5 | EutG |
| 4 | 4 | 0.5 | 0.5 | YiaY |
| 5 | 4 | 0.5 | 0.5 | AdhP |
| 6 | 4 | 0.5 | 0.5 | Lpd |
| 7 | 4 | 0.5 | 0.5 | AceE |
