Revision as of 20:59, 1 November 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

09/01/2017 PCR Primer

MgCl:

???	???	???
14.3	H2O	114.4
2.5	Buffer	20
1	MgCl2	8
1	dNTP	8

tube:

2ul	H2O
-5ul	F
-5ul	R

Label:

0	Control
1	AceF
2	EutG BB
3	YiaY BB
4	Adhp BB
5	Lpol
6	AceE

09/06/2017 Genomic PCR

Master Mix

gDNA	3	24
Buffer	2.5	20
???	1	8
dH2O	13.3	106.4
???	0.2	1.6

/	dH2O	For	Rev	/
1	4	0.5	0.5	AceF
2	4	0.5	0.5	EutG
3	4	0.5	0.5	YiaY
4	4	0.5	0.5	AdnP
5	4	0.5	0.5	Lpd
6	4	0.5	0.5	AceE
7	5	-	-	-

09/11/2017 Genomic PCR

Master Mix

gDNA	3	24
Buffer	2.5	20
dNTP	1	8
dH2O	12.3	98.4
taq	0.2	1.6
MgCl2	1	8

	dH2O	For	Rev
0	5	-	-
1	4	0.5	0.5	ACE F
2	4	0.5	0.5	EutG
3	4	0.5	0.5	YiaY
4	4	0.5	0.5	AdhP
5	4	0.5	0.5	Lpd
6	4	0.5	0.5	AceE

09/18/2017 PCR check of J23102 and GlmY

1	-	DNA(0)	dH2O(5)
2	J23102	3	2
3	GlmY	3	2
4	A(1)	3	2
5/td>	A(2)	3	2
6	B(2)	3	2
7	C(2)	3	2

Max

Buffer	2.5	20
dNTP	1	8
VR	0.5	4
VF2	0.5	4
taq	0.2	1.6
dH2O	15.3	122.4

09/25/2017 PCR check for J23102+GlmY

Master Mix

Buffer	2.5	20
dNTP	1	8
VR	0.5	4
VF2	0.5	4
taq	0.2	1.6
dH2O	15.3	122.4

		DNA	H2O
1	Control	0u	5u
2	J231013	3	2
3	GlmY-3A	3	2
4	2C	3	2
5	1C	3	2
6	1B	3	2
7	1A	3	2

10/10/2017 PCR check for J23102+GlmY

Master Mix

Buffer	2.5	20
dNTP	1	8
VR	0.5	4
VF2	0.5	4
taq	0.2	1.6
dH2O	15.3	122.4

		DNA	H2O
1	Control	0u	5u
2	J231013	3	2
3	GlmY-3A	3	2
4	2C	3	2
5	1C	3	2
6	1B	3	2
7	1A	3	2

10/20/2017 PCR J23102+GlmY

Master Mix

Buffer	2.5	20
dNTP	1	8
VR	0.5	4
VF2	0.5	4
taq	0.2	1.6
dH2O	15.3	122.4

		DNA	H2O
1	Control	0u	5u
2	J23102B pp	3	2
3	J23102 P NNP	3	2
4	GlmY-3A	3	2
5	102B+GlmY 1B	3	2
6	102B+GlmY 1C	3	2
7	102B_GlmY 1D	3	2

10/23/2017 Genomic PCR

Master Mix

H2O	13.3	106.4
Buffer	2.5	20
dNTP	1	8
GDNA	3	24
taq	0.2	1.6

Gel:

	dH2O	For	Rev
1	5	-	-
2	4	0.5	0.5	AceF
3	4	0.5	0.5	EutG
4	4	0.5	0.5	YiaY
5	4	0.5	0.5	AdhP
6	4	0.5	0.5	Lpd
7	4	0.5	0.5	AceE

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Revision as of 20:59, 1 November 2017

Notebook