Difference between revisions of "Team:TecCEM/Demonstrate"

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                <h1 class = "titleRed">Demonstrate</h1>
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                    <h1 class = "titleRed">Demonstrate</h1>
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                    <h1 class = "subTitleUbuntu">Math modeling with our results</h1>
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                <h1 class = "subTitleUbuntu">Math modeling with our results</h1>
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                    <p></br>We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The <span class="italicText">d</span> and <span class="italicText">θ</span> were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.</br></br></p>
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                <p></br>We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The <span class="italicText">d</span> and <span class="italicText">θ</span> were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.</br></br></p>
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                    <img class = "centerImage"src="https://static.igem.org/mediawiki/2017/3/3f/TEC-CEM_grafico_demonstrate.jpg">
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                    <p></br>The following table shows the values for each point using the equation.</br></br></p>
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                            <th> X</th>
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                            <th >Xm </th>
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                <p></br>The following table shows the values for each point using the equation.</br></br></p>
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                            <th >T-Xm </th>
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<th> X</th>
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                            <th> 0 </th>
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<th >Xm </th>
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                            <th >1 </th>
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                            <th >1 </th>
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                            <th> 20</th>
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                            <th> 0.9 </th>
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                            <th >0.97992</th>
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                            <th> 40</th>
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                            <th> 0.6</th>
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                            <th> 0.632184</th>
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<th >0.97992</th>
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                            <th> 60 </th>
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                            <th> 0.57 </th>
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                            <th> 0.285714 </th>
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<th> 0.632184</th>
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                            <th> 100</th>
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                            <th> 0.25</th>
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                            <th> 0.177328</th>
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<th> 0.285714 </th>
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<th> 0.25</th>
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<th> 0.177328</th>
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                                <h1 class = "subTitleUbuntu">siRNA confirmation</h1>
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                                <p></br>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus efficitur dolor erat, vel lobortis augue mattis nec. Ut sit amet placerat massa. Sed dignissim ante eget nibh sollicitudin, at tincidunt mi fermentum. Curabitur tempus nibh in velit maximus egestas. Sed massa ipsum, maximus at dictum dapibus, convallis eget augue. Cras purus mauris, mattis quis ornare a, porttitor non quam. Donec sem felis, feugiat vitae porta sit amet, laoreet a leo. Proin in arcu iaculis, facilisis nisi at, rutrum neque. Nullam condimentum, urna quis pharetra lacinia, justo quam fermentum augue, at porta turpis turpis aliquet risus. Aenean lacinia nunc eu porttitor aliquet. Aenean mattis posuere felis, ac finibus est sodales sit amet. Integer lobortis metus vitae ante sollicitudin pharetra. Quisque egestas sem quis ante tristique cursus. Mauris non blandit velit. Ut euismod ut risus rutrum aliquam.</br></br></p>
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                <p></br>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus efficitur dolor erat, vel lobortis augue mattis nec. Ut sit amet placerat massa. Sed dignissim ante eget nibh sollicitudin, at tincidunt mi fermentum. Curabitur tempus nibh in velit maximus egestas. Sed massa ipsum, maximus at dictum dapibus, convallis eget augue. Cras purus mauris, mattis quis ornare a, porttitor non quam. Donec sem felis, feugiat vitae porta sit amet, laoreet a leo. Proin in arcu iaculis, facilisis nisi at, rutrum neque. Nullam condimentum, urna quis pharetra lacinia, justo quam fermentum augue, at porta turpis turpis aliquet risus. Aenean lacinia nunc eu porttitor aliquet. Aenean mattis posuere felis, ac finibus est sodales sit amet. Integer lobortis metus vitae ante sollicitudin pharetra. Quisque egestas sem quis ante tristique cursus. Mauris non blandit velit. Ut euismod ut risus rutrum aliquam.</br></br></p>
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Revision as of 21:25, 1 November 2017

IGEM_TECCEM

Demonstrate

Demonstrate

Math modeling with our results


We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The d and θ were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.


The following table shows the values for each point using the equation.

X Xm T-Xm
0 1 1
20 0.9 0.97992
40 0.6 0.632184
60 0.57 0.285714
100 0.25 0.177328

siRNA confirmation


Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus efficitur dolor erat, vel lobortis augue mattis nec. Ut sit amet placerat massa. Sed dignissim ante eget nibh sollicitudin, at tincidunt mi fermentum. Curabitur tempus nibh in velit maximus egestas. Sed massa ipsum, maximus at dictum dapibus, convallis eget augue. Cras purus mauris, mattis quis ornare a, porttitor non quam. Donec sem felis, feugiat vitae porta sit amet, laoreet a leo. Proin in arcu iaculis, facilisis nisi at, rutrum neque. Nullam condimentum, urna quis pharetra lacinia, justo quam fermentum augue, at porta turpis turpis aliquet risus. Aenean lacinia nunc eu porttitor aliquet. Aenean mattis posuere felis, ac finibus est sodales sit amet. Integer lobortis metus vitae ante sollicitudin pharetra. Quisque egestas sem quis ante tristique cursus. Mauris non blandit velit. Ut euismod ut risus rutrum aliquam.

           


	
	
	IGEM_TECCEM