Difference between revisions of "Team:Hamburg/Lab Results"
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Biochemistry | Biochemistry | ||
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| − | In this project we were able to produce several parts, but unfortunately we only had enough time to charcterize some of them. To these belong promotor + tired + terminator and promtor + sfp, which were charcterized via kinetic measurements. The measured OD values before and after induction with 400 mM IPTG (+ 200 µg cystein) are shown in tables 1 and 2. </ | + | In this project we were able to produce several parts, but unfortunately we only had enough time to charcterize some of them. To these belong promotor + tired + terminator and promtor + sfp, which were charcterized via kinetic measurements. The measured OD values before and after induction with 400 mM IPTG (+ 200 µg cystein) are shown in tables 1 and 2. </p> |
Table 1: OD values before induction | Table 1: OD values before induction | ||
<table border=2> | <table border=2> | ||
Revision as of 21:33, 1 November 2017
Template:Team-:Hamburg/CSS
Lab Results
Biochemistry
In this project we were able to produce several parts, but unfortunately we only had enough time to charcterize some of them. To these belong promotor + tired + terminator and promtor + sfp, which were charcterized via kinetic measurements. The measured OD values before and after induction with 400 mM IPTG (+ 200 µg cystein) are shown in tables 1 and 2.
Table 1: OD values before induction| BL21 | tired | sfp | |
|---|---|---|---|
| t = 0 | 0.1250 | 0.0364 | 0.0260 |
| t = 70 | 0.3473 | 0.0644 | 0.1154 |
| t = 120 | 1.0422 | 0.3031 | 0.4058 |
| t = 180 | 1.5018 | 0.4356 | 0.9930 |
Chemistry
Nanoscience
Sadly our first charge of microfluidic devices broke down when we tried to coat them with an ECM-solution to grow our cells on. And we were not able to produce a second charge before wiki freeze.
